Remarkable recent changes in genetic diversity of the avirulence gene AvrStb6 in global populations of the wheat pathogen Zymoseptoria tritici
Remarkable recent changes in genetic diversity of the avirulence gene AvrStb6 in global populations of the wheat pathogen Zymoseptoria tritici
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小麦病原菌小麦发酵斑孢菌全球群体中无毒基因 AvrStb6 遗传多样性近期发生显着变化
DOI：
10.1101/2020.09.18.303370
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Stephens C
中科院分区：
文献类型：
--
作者：
Stephens C
小麦黑斑病（STB）是由小麦酵母（zymoseptoria tritici）引起的小麦病害，是小麦最重要的经济病害之一。最近，这两个因素之间的基因对基因相互作用。小麦，小麦受体样激酶Stb6和theZ。小麦分泌效应蛋白AvrStb6已被鉴定。先前的分析显示avrstb6单倍型在早期z中存在高度多样性。在收集的tritriisolate中，高达0.18%的分析分离株具有与最初在参考分离株IPO323中鉴定的AvrStb6相同的无毒同种异构体。由于stb6存在于全球许多商品小麦品种中，我们旨在评估avrstb6遗传多样性的潜在变化，以及在最近的z .小麦群体中允许逃避stb6介导抗性的单倍型的发生率。通过对avrstb6进行定向重测序，我们发现该基因普遍存在于2013-2017年世界主要小麦产区的田间分离株中。然而，与先前AvrStb6群体研究的数据相反，我们报告在现代z . tritritis分离株中完全没有最初描述的AvrStb6的无毒亚型。此外，在现代小麦分离株中发现了数量非常少的单倍型，每种单倍型编码的AvrStb6蛋白同工型调节了对含有stb6的小麦的毒力。发现AvrStb6的单一毒力异构体在全球种群中特别丰富。这些发现表明，尽管z。在抗性宿主上有性繁殖的小虫，avrstb6毒性单倍型在随后的种群中倾向于被消除。
Septoria tritici blotch (STB), caused by the fungusZymoseptoria tritici, is one of the most economically important diseases of wheat. Recently, both factors of a gene‐for‐gene interaction betweenZ.triticiand wheat, the wheat receptor‐like kinase Stb6 and theZ.triticisecreted effector protein AvrStb6, have been identified. Previous analyses revealed a high diversity ofAvrStb6haplotypes present in earlierZ.triticiisolate collections, with up to c.18% of analysed isolates possessing the avirulence isoform of AvrStb6 identical to that originally identified in the reference isolate IPO323. WithStb6present in many commercial wheat cultivars globally, we aimed to assess potential changes inAvrStb6genetic diversity and the incidence of haplotypes allowing evasion ofStb6‐mediated resistance in more recentZ.triticipopulations. Here we show, using targeted resequencing ofAvrStb6, that this gene is universally present in field isolates sampled from major wheat‐growing regions of the world in 2013–2017. However, in contrast to the data from previousAvrStb6population studies, we report a complete absence of the originally described avirulence isoform of AvrStb6 amongst modernZ.triticiisolates. Moreover, a remarkably small number of haplotypes, each encoding AvrStb6 protein isoforms conditioning virulence onStb6‐containing wheat, were found to predominate among modernZ.triticiisolates. A single virulence isoform of AvrStb6 was found to be particularly abundant throughout the global population. These findings indicate that, despite the ability ofZ.triticito sexually reproduce on resistant hosts,AvrStb6avirulence haplotypes tend to be eliminated in subsequent populations.
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影响因子：
4
作者：
Dong, Suomeng;Raffaele, Sylvain;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
影响因子：
4.5
作者：
Soyer JL;El Ghalid M;Glaser N;Ollivier B;Linglin J;Grandaubert J;Balesdent MH;Connolly LR;Freitag M;Rouxel T;Fudal I
通讯作者：
Fudal I
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
4.4
作者：
McDonald, Megan C.;Renkin, Melanie;Milgate, Andrew
通讯作者：
Milgate, Andrew
DOI：
10.1007/978-1-0716-0356-7_12
发表时间：
2020-01-01
期刊：
BIOLISTIC DNA DELIVERY IN PLANTS
影响因子：
--
作者：
Sparks, Caroline A.;Doherty, Angela
通讯作者：
Doherty, Angela