Neurotensin co-expressed in orexin-producing neurons in the lateral hypothalamus plays an important role in regulation of sleep/wakefulness states.
Neurotensin co-expressed in orexin-producing neurons in the lateral hypothalamus plays an important role in regulation of sleep/wakefulness states.
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DOI：
10.1371/journal.pone.0062391
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sakurai T
中科院分区：
文献类型：
--
作者：
Furutani N;Hondo M;Kageyama H;Tsujino N;Mieda M;Yanagisawa M;Shioda S;Sakurai T
食欲素和神经降压素都在下丘脑外侧区(LHA)表达，并参与调节摄食、运动活动和奖赏系统。双标记免疫荧光和原位杂交研究表明，神经降压素和增食欲素共存于LHA的神经元中。药理学研究表明，神经降压素通过激活神经降压素受体-2(NTSR-2)和非选择性阳离子通道来兴奋产生食欲素的神经元(食欲素神经元)。原位杂交结果显示，大多数增食欲素神经元表达神经降压素受体-2，而不表达神经降压素受体-1(NTSR-1)。免疫组织化学研究表明，神经降压素免疫反应纤维与增食欲素神经元相对应。一种神经降压素受体拮抗剂可以减少野生型小鼠体内增食欲素神经元的Fos表达和觉醒时间。然而，该拮抗剂对食欲素神经元消融小鼠的这些参数没有任何影响。这些观察表明，神经降压素在维持食欲素神经元活动中的重要性。这里提出的证据扩大了我们对食欲素神经元调控机制的理解。
Both orexin and neurotensin are expressed in the lateral hypothalamic area (LHA) and have been implicated in the regulation of feeding, motor activity and the reward system. A double label immunofluorescence and in situ hybridization studies showed that neurotensin colocalizes with orexin in neurons of the LHA. Pharmacological studies suggested that neurotensin excites orexin-producing neurons (orexin neurons) through activation of neurotensin receptor-2 (NTSR-2) and non-selective cation channels. In situ hybridization study showed that most orexin neurons express neurotensin receptor-2 mRNA but not neurotensin receptor-1 (Ntsr-1) mRNA. Immunohistochemical studies showed that neurotensin-immunoreactive fibers make appositions to orexin neurons. A neurotensin receptor antagonist decreased Fos expression in orexin neurons and wakefulness time in wild type mice when administered intraperitoneally. However, the antagonist did not evoke any effect on these parameters in orexin neuron-ablated mice. These observations suggest the importance of neurotensin in maintaining activity of orexin neurons. The evidence presented here expands our understanding of the regulatory mechanism of orexin neurons.
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影响因子：
4.2
作者：
Dicou, E;Vincent, JP;Mazella, J
通讯作者：
Mazella, J
DOI：
10.1073/pnas.96.2.748
发表时间：
1999-01-19
影响因子：
11.1
作者：
Date, Y;Ueta, Y;Nakazato, M
通讯作者：
Nakazato, M
影响因子：
--
作者：
Ferraro, Luca;Tomasini, Maria C.;Antonelli, Tiziana
通讯作者：
Antonelli, Tiziana
影响因子：
3.3
作者：
HOSLI, E;STAUFFER, S;HOSLI, L
通讯作者：
HOSLI, L
影响因子：
5.3
作者：
Martin, S;Vincent, JP;Mazella, J
通讯作者：
Mazella, J