Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa)
Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa)
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杨树CDPK及其密切相关基因家族的全基因组鉴定、分类和表达分析
DOI：
10.1007/s11033-012-2351-z
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发表时间：
2012-12
影响因子：
2.8
通讯作者：
Zhou, Gongke
中科院分区：
文献类型：
--
作者：
Zuo, Ran;Hu, Ruibo;Chai, Guohua;Xu, Meiling;Qi, Guang;Kong, Yingzhen;Zhou, Gongke
钙依赖蛋白激酶(CDPKs)是一类钙离子结合蛋白，在整个植物界和某些类型的原生生物中发现的钙信号转导途径中发挥着重要作用。在拟南芥、水稻和小麦中已经进行了CDPKs的全基因组分析，相当多的CDPKs被证明在植物胁迫响应信号通路中发挥关键作用。本研究对杨树CDPK及其亲缘关系密切的基因家族进行了全面的分析，包括系统发育、染色体位置、基因结构和表达谱。共鉴定出30个杨树CDPK1基因和20个亲缘关系密切的激酶基因，它们在系统发育上分为8个亚科，主要分布在15个连锁群上。基因组组织分析表明，纯化选择在杨树CDPK基因家族的保留和维持中起着关键作用。此外，基因芯片分析表明，许多杨树CDK及其密切相关基因在不同的组织和不同的胁迫下存在差异表达。此外，还研究了近缘基因对的表达谱，以揭示它们的进化命运。此外，还对所选择的9个CDPKs基因进行了实时定量RT-PCR，以确定它们对干旱胁迫的响应。这些观察结果可能为今后对杨树CDPK及其密切相关基因家族进行功能分析以揭示其生物学功能奠定基础。
Calcium-dependent protein kinases (CDPKs) are Ca2+-binding proteins known to play crucial roles in Ca2+signal transduction pathways which have been identified throughout plant kingdom and in certain types of protists. Genome-wide analysis of CDPKs have been carried out inArabidopsis, rice and wheat, and quite a few of CDPKs were proved to play crucial roles in plant stress responsive signature pathways. In this study, a comprehensive analysis ofPopulusCDPK and its closely related gene families was performed, including phylogeny, chromosome locations, gene structures, and expression profiles. ThirtyPopulus CDPKgenes and twenty closely related kinase genes were identified, which were phylogenetically clustered into eight distinct subfamilies and predominately distributed across fifteen linkage groups (LG). Genomic organization analyses indicated that purifying selection has played a pivotal role in the retention and maintenance ofPopulusCDPK gene family. Furthermore, microarray analysis showed that a number ofPopulus CDPKand its closely related genes differentially expressed across disparate tissues and under various stresses. The expression profiles of paralogous pairs were also investigated to reveal their evolution fates. In addition, quantitative real-time RT-PCR was performed on nine selectedCDPKgenes to confirm their responses to drought stress treatment. These observations may lay the foundation for future functional analysis ofPopulusCDPK and its closely related gene families to unravel their biological roles.
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DOI：
10.1007/978-3-642-20829-4
发表时间：
2011
期刊：
--
影响因子：
--
作者：
S. Luan
通讯作者：
S. Luan
影响因子：
7.4
作者：
Jun-Jie Zou;Fengjie Wei;Cun Wang;Juan Wu;D. Ratnasekera;Wen-Xin Liu;Wei-Hua Wu
通讯作者：
Jun-Jie Zou;Fengjie Wei;Cun Wang;Juan Wu;D. Ratnasekera;Wen-Xin Liu;Wei-Hua Wu
影响因子：
2.9
作者：
Wernimont, Amy K.;Amani, Merhnaz;Hui, Raymond
通讯作者：
Hui, Raymond
影响因子：
5.1
作者：
Zhihua Liu-;M. Xia;B. Poovaiah
通讯作者：
Zhihua Liu-;M. Xia;B. Poovaiah
盐和干旱诱导的杨树 GRAS 蛋白 SCL7 赋予拟南芥耐盐和干旱的能力
影响因子：
6.9
作者：
Ma HS;Liang D;Shuai P;Xia XL;Yin WL
通讯作者：
Yin WL