Identification and functional characterization of the sex-determining gene doublesex in the sawfly, Athalia rosae (Hymenoptera: Tenthredinidae).
Identification and functional characterization of the sex-determining gene doublesex in the sawfly, Athalia rosae (Hymenoptera: Tenthredinidae).
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DOI：
10.1007/s13355-017-0502-3
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发表时间：
2017
影响因子：
1.3
通讯作者：
Suzuki MG
中科院分区：
文献类型：
--
作者：
Mine S;Sumitani M;Aoki F;Hatakeyama M;Suzuki MG
性命运的叶蜂，Athalia rosae（膜翅目：叶蜂科）是由互补性决定（CSD）机制，是在蜜蜂的情况下。然而，到目前为止，在这个物种中还没有确定性别决定的基因。在这项研究中，我们试图从A. rosae基因组中鉴定互补性别决定因子（csd）、雌性化因子（fem）和doublemex（dsx）的直系同源物，所有这些都是蜜蜂性别决定级联的关键组成部分。结果，我们鉴定了dsx的叶蜂直系同源物（指定为Ardsx）。cDNA末端的快速扩增（RACE）使用从雄性和雌性幼虫中提取的总RNA鉴定出三种雄性特异性变体和三种雌性特异性变体。全长Ardsx cDNA与基因组序列之间的比较显示，男性和女性特异性变体之间的外显子5发生了差异拼接。RT-PCR分析表明，Ardsx前mRNA的剪接在几乎所有的发育阶段的性别依赖的方式。RNAi介导的敲除雄性中的Ardsx引起生殖器官的严重缺陷，并且特别地，诱导含有背对叶片和鞘的产卵器的发育。这些雄性还显示睾丸和精囊异常，缺乏成熟精子。目前的研究提供了第一个直接证据，dsx是必不可少的性发育在翅目物种。
Sexual fate of the sawfly, Athalia rosae (Hymenoptera: Tenthredinidae) is determined by the complementary sex determination (CSD) mechanism as is the case in honeybees. However, to date, genes involved in sex determination have not been identified in this species. In this study, we attempted to identify orthologs of complementary sex-determiner (csd), feminizer (fem), and doublesex (dsx) from the A. rosae genome, all of which are crucial components of the sex determination cascade in the honeybee. As a result, we identified a sawfly ortholog of dsx (designated as Ardsx). Rapid amplification of cDNA ends (RACE) using total RNA extracted from male and female larvae identified three male-specific variants and three female-specific variants. Comparison between the full-length Ardsx cDNAs and the genomic sequence revealed that exon 5 was differentially spliced between the male- and female-specific variants. RT-PCR analysis demonstrated that Ardsx pre-mRNA was spliced alternatively in a sex-dependent manner at almost all the developmental stages. RNAi-mediated knockdown of Ardsx in males caused severe defects in the reproductive organs and, notably, induced development of the ovipository apparatus containing the dorsal pair of blades and the sheath. These males also showed abnormalities in testes and seminal vesicles and lacked mature sperm. The present study provides the first direct evidence that dsx is essential for sexual development in hymenopteran species.
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影响因子：
56.9
作者：
HOSHIJIMA, K;INOUE, K;SHIMURA, Y
通讯作者：
SHIMURA, Y
DOI：
10.1126/science.aaa2850
发表时间：
2015-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hall AB;Basu S;Jiang X;Qi Y;Timoshevskiy VA;Biedler JK;Sharakhova MV;Elahi R;Anderson MA;Chen XG;Sharakhov IV;Adelman ZN;Tu Z
通讯作者：
Tu Z
影响因子：
0.8
作者：
HATAKEYAMA, M;SAWA, M;OISHI, K
通讯作者：
OISHI, K
影响因子：
3.3
作者：
Cho, Soochin;Huang, Zachary Y.;Zhang, Jianzhi
通讯作者：
Zhang, Jianzhi
影响因子：
64.8
作者：
Hasselmann, Martin;Gempe, Tanja;Beye, Martin
通讯作者：
Beye, Martin