Homeostasis of cell wall integrity pathway phosphorylation is required for the growth and pathogenicity of Magnaporthe oryzae.
Homeostasis of cell wall integrity pathway phosphorylation is required for the growth and pathogenicity of Magnaporthe oryzae.
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DOI：
10.1111/mpp.13225
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发表时间：
2022-08
影响因子：
4.9
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Cai Y;Liu X;Shen L;Wang N;He Y;Zhang H;Wang P;Zhang Z
细胞壁为抵御外部环境施加的压力提供了重要的屏障。在稻瘟病菌 Magnaporthe oryzae 中，这种应激反应是由细胞壁完整性 (CWI) 途径介导的，该途径由一个充分表征的蛋白质磷酸化级联组成。然而，维持 CWI 磷酸化稳态的其他调节因子，例如蛋白磷酸酶 (PPase)，仍不清楚。在这里，我们鉴定了两种 PPase：MoPtc1 和 MoPtc2，它们充当 CWI 途径的负调节因子。 MoPtc1 和 MoPtc2 与 MoMkk1 相互作用，MoMkk1 是 CWI 途径的关键组成部分之一，对于米霉的营养生长、分生孢子形成和毒力至关重要。我们还证明 MoPtc1 和 MoPtc2 在体内和体外都能使 MoMkk1 去磷酸化，并且 CWI 应激会导致 MoPtc1 和 MoMkk1 之间的相互作用增强。 CWI 应激消除了 MoPtc2 和 MoMkk1 之间的相互作用，为 CWI 信号传导提供了一种失活方法。我们的研究表明，米霉中的 CWI 信号传导是一种高度协调的调节机制，对于应激反应和致病性至关重要。这项研究揭示了 MoPtc1 和 MoPtc2 通过去磷酸化调节 MoMkk1 和细胞壁完整性信号传导以对抗 Magnaporthe oryzae 细胞壁应激的新机制。
The cell wall provides a crucial barrier to stress imposed by the external environment. In the rice blast fungus Magnaporthe oryzae, this stress response is mediated by the cell wall integrity (CWI) pathway, consisting of a well‐characterized protein phosphorylation cascade. However, other regulators that maintain CWI phosphorylation homeostasis, such as protein phosphatases (PPases), remain unclear. Here, we identified two PPases, MoPtc1 and MoPtc2, that function as negative regulators of the CWI pathway. MoPtc1 and MoPtc2 interact with MoMkk1, one of the key components of the CWI pathway, and are crucial for the vegetative growth, conidial formation, and virulence of M. oryzae. We also demonstrate that both MoPtc1 and MoPtc2 dephosphorylate MoMkk1 in vivo and in vitro, and that CWI stress leads to enhanced interaction between MoPtc1 and MoMkk1. CWI stress abolishes the interaction between MoPtc2 and MoMkk1, providing a means of deactivation for CWI signalling. Our studies reveal that CWI signalling in M. oryzae is a highly coordinated regulatory mechanism vital for stress response and pathogenicity. This study reveals a novel mechanism of MoPtc1 and MoPtc2 in regulating MoMkk1 and cell wall integrity signalling by dephosphorylation to counter cell wall stress in Magnaporthe oryzae.
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