Susceptibility of Caenorhabditis elegans to Burkholderia infection depends on prior diet and secreted bacterial attractants.
Susceptibility of Caenorhabditis elegans to Burkholderia infection depends on prior diet and secreted bacterial attractants.
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DOI：
10.1371/journal.pone.0007961
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发表时间：
2009-11-23
期刊：
影响因子：
3.7
通讯作者：
Lipuma JJ
中科院分区：
文献类型：
--
作者：
Cooper VS;Carlson WA;Lipuma JJ
线虫秀丽线虫可被某些病原菌杀死，是研究细菌与动物宿主相互作用的模式生物。然而，在猎物细菌上生长线虫可能会影响它们对潜在病原体的敏感性。建立了一种无菌线虫培养方法来分离和定量线虫与洋葱伯克霍尔德菌复合体潜在致病菌株之间的相互作用。在液体溶液中而不是在琼脂表面上研究这些动态，可以最大限度地减少线虫的回避行为，并解决不同菌株之间的更多差异。大多数B.cenocepacia、B.ambiaria和B.cepacia菌株在7天后对线虫的致死率为60-80%，而多食芽孢杆菌的致死率较低(25%)，并支持线虫繁殖。然而，一些从慢性感染中恢复的线虫分离株的毒力要低得多(5-28%的死亡率)。正如预测的那样，先前的饮食改变了线虫和细菌之间相互作用的结果。当让伯克霍尔德氏菌和大肠杆菌作为琼脂上的猎物进行选择时，轴向生长的线虫最初更喜欢以伯克霍尔德氏菌为食物来源，而不是以大肠杆菌为食物来源，但对于喂食大肠杆菌的线虫来说情况并非如此，因为它避免了有毒的伯克霍尔德氏菌。这种食物偏好与无细胞培养上清液相关，因此分泌的化合物可能介导了细菌-线虫的相互作用。这个模型将细菌和线虫之间的相互作用与先前摄食的影响隔离开来，表明细菌可以影响线虫的行为及其对病原体的敏感性。
The nematode Caenorhabditis elegans may be killed by certain pathogenic bacteria and thus is a model organism for studying interactions between bacteria and animal hosts. However, growing nematodes on prey bacteria may influence their susceptibility to potential pathogens. A method of axenic nematode culture was developed to isolate and quantify interactions between C. elegans and potentially pathogenic strains of the Burkholderia cepacia complex. Studying these dynamics in liquid solution rather than on agar surfaces minimized nematode avoidance behavior and resolved more differences among isolates. Most isolates of B. cenocepacia, B. ambifaria and B. cepacia caused 60–80% mortality of nematodes after 7 days, whereas isolates of B. multivorans caused less mortality (<25%) and supported nematode reproduction. However, some B. cenocepacia isolates recovered from chronic infections were much less virulent (5–28% mortality). As predicted, prior diet altered the outcome of interactions between nematodes and bacteria. When given the choice between Burkholderia and E. coli as prey on agar, axenically raised nematodes initially preferred most lethal Burkholderia isolates to E. coli as a food source, but this was not the case for nematodes fed E. coli, which avoided toxic Burkholderia. This food preference was associated with the cell-free supernatant and thus secreted compounds likely mediated bacterial-nematode interactions. This model, which isolates interactions between bacteria and nematodes from the effects of prior feeding, demonstrates that bacteria can influence nematode behavior and their susceptibility to pathogens.
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影响因子：
9.4
作者：
Coenye, T;Spilker, T;LiPuma, JJ
通讯作者：
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影响因子：
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