Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model.
Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model.
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膳食补充剂干酪乳杆菌可减轻猪模型中脂多糖引起的肝损伤。
DOI：
10.3390/ijms18122535
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发表时间：
2017-11-26
影响因子：
5.6
通讯作者：
Wu G
中科院分区：
文献类型：
--
作者：
Zhao D;Wu T;Yi D;Wang L;Li P;Zhang J;Hou Y;Wu G
本研究旨在确定干酪乳杆菌（L. casei）是否可以缓解脂多糖（LPS）攻击仔猪的肝损伤。仔猪被随机分配到三组中的一组：对照组、LPS 组和干酪乳杆菌组。对照组和LPS组饲喂以玉米和豆粕为主的饮食，而干酪乳杆菌组饲喂基础饮食并补充6×106 cfu/g干酪乳杆菌。试验第31天，LPS组和干酪乳杆菌组的仔猪腹腔注射LPS（100μg/kg体重），而对照组则注射相同体积的生理盐水。收集血液和肝脏样本进行分析。结果表明，补充干酪乳杆菌可降低料重比 (p = 0.027) 和腹泻发生率 (p < 0.001)，并减轻 LPS 诱导的肝脏组织形态学异常。与对照组相比，LPS 攻击显着增加了血浆中谷氨酰转肽酶活性 (p = 0.001) 以及肝脏中白细胞介素 6 (IL-6) (p = 0.048)、肿瘤坏死因子-α (TNF-α) (p = 0.041) 和丙二醛 (MDA) (p = 0.001) 的浓度，同时降低了肝脏 SOD 活性。 LPS 还增加 (p < 0.05) 肝脏中 IL-6、IL-8、TNF-α、Toll 样受体 4 (TLR4)、核因子 κB (NF-κB) 和热休克蛋白 70 (HSP70) 的 mRNA 水平。补充干酪乳杆菌可改善 LPS 攻击的不利影响。总之，膳食干酪乳杆菌通过减少促炎细胞因子和增加抗氧化能力来减轻 LPS 引起的肝损伤。
This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas the L. casei group was fed the basal diet supplemented with 6 × 106 cfu/g L. casei. On Day 31 of the trial, piglets in the LPS and L. casei groups received intraperitoneal administration of LPS (100 µg/kg body weight), while the control group received the same volume of saline. Blood and liver samples were collected for analysis. Results showed that L. casei supplementation decreased the feed/gain ratio (p = 0.027) and diarrhea incidence (p < 0.001), and attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, LPS challenge dramatically increased glutamyl transpeptidase activity (p = 0.001) in plasma as well as the concentrations of Interleukin 6 (IL-6) (p = 0.048), Tumor necrosis factor-alpha (TNF-α) (p = 0.041), and Malondialdehyde (MDA) (p = 0.001) in the liver, while decreasing the hepatic SOD activity. LPS also increased (p < 0.05) the mRNA levels for IL-6, IL-8, TNF-α, Toll-like receptors 4 (TLR4), Nuclear factor κB (NF-κB) and Heat shock protein 70 (HSP70) in the liver. The adverse effects of LPS challenge were ameliorated by L. casei supplementation. In conclusion, dietary L. casei alleviates LPS-induced liver injury via reducing pro-inflammatory cytokines and increasing anti-oxidative capacity.
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作者：
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