Characteristics of Anti-SARS-CoV-2 Antibodies in Recovered COVID-19 Subjects.
Characteristics of Anti-SARS-CoV-2 Antibodies in Recovered COVID-19 Subjects.
复制标题
COVID-19感染者的抗SARS-CoV-2抗体特征。
DOI：
10.3390/v13040697
复制
发表时间：
2021-04-16
期刊：
影响因子：
--
通讯作者：
Nazy I
中科院分区：
文献类型：
--
作者：
Huynh A;Arnold DM;Smith JW;Moore JC;Zhang A;Chagla Z;Harvey BJ;Stacey HD;Ang JC;Clare R;Ivetic N;Chetty VT;Bowdish DME;Miller MS;Kelton JG;Nazy I
2019冠状病毒病（COVID-19）是由新型严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）引起的全球大流行疾病。虽然目前采用逆转录聚合酶链反应（RT-PCR）检测SARS-CoV-2用于诊断COVID-19急性感染，但需要进行血清学检测来研究SARS-CoV-2的体液免疫反应。采用酶联免疫吸附试验（ELISA）对153例rt - pcr阳性和55例rt - pcr阴性的康复受试者进行了抗sars - cov -2免疫球蛋白（Ig）G/A/M抗体及其受体结合域（RBD）的检测。还进一步评估了这些抗体中和活的SARS-CoV-2病毒的能力。在症状出现后180天内，90.9%的痊愈受试者检测到抗sars - cov -2抗体。抗s蛋白和抗rbd IgG滴度与病毒中和相关（r = 0.5157和r = 0.6010）。在rt - pcr阳性的受试者中，22人（14.3%）没有抗sars - cov -2抗体；其中17例具有RT-PCR周期阈值（Ct） bbb27。这些高Ct值增加了这些不确定结果来自未感染或轻度感染的个体未能引起抗体反应的可能性。这项研究强调了血清学调查的重要性，以确定基于RT-PCR确定的感染数量的人群水平免疫。
Coronavirus Disease 2019 (COVID-19) is a global pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While detection of SARS-CoV-2 by polymerase chain reaction with reverse transcription (RT-PCR) is currently used to diagnose acute COVID-19 infection, serological assays are needed to study the humoral immune response to SARS-CoV-2. Anti-SARS-CoV-2 immunoglobulin (Ig)G/A/M antibodies against spike (S) protein and its receptor-binding domain (RBD) were characterized in recovered subjects who were RT-PCR-positive (n = 153) and RT-PCR-negative (n = 55) using an enzyme-linked immunosorbent assay (ELISA). These antibodies were also further assessed for their ability to neutralize live SARS-CoV-2 virus. Anti-SARS-CoV-2 antibodies were detected in 90.9% of resolved subjects up to 180 days post-symptom onset. Anti-S protein and anti-RBD IgG titers correlated (r = 0.5157 and r = 0.6010, respectively) with viral neutralization. Of the RT-PCR-positive subjects, 22 (14.3%) did not have anti-SARS-CoV-2 antibodies; and of those, 17 had RT-PCR cycle threshold (Ct) values > 27. These high Ct values raise the possibility that these indeterminate results are from individuals who were not infected or had mild infection that failed to elicit an antibody response. This study highlights the importance of serological surveys to determine population-level immunity based on infection numbers as determined by RT-PCR.
登录
查看更多内容
影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
DOI：
10.1093/cid/ciaa1616
发表时间：
2021-08-16
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Gniazdowski V;Paul Morris C;Wohl S;Mehoke T;Ramakrishnan S;Thielen P;Powell H;Smith B;Armstrong DT;Herrera M;Reifsnyder C;Sevdali M;Carroll KC;Pekosz A;Mostafa HH
通讯作者：
Mostafa HH
影响因子：
3.7
作者：
Marklund E;Leach S;Axelsson H;Nyström K;Norder H;Bemark M;Angeletti D;Lundgren A;Nilsson S;Andersson LM;Yilmaz A;Lindh M;Liljeqvist JÅ;Gisslén M
通讯作者：
Gisslén M
DOI：
10.1007/s10096-020-03913-9
发表时间：
2020-04-27
影响因子：
4.5
作者：
La Scola, Bernard;Le Bideau, Marion;Raoult, Didier
通讯作者：
Raoult, Didier
影响因子：
82.9
作者：
He, Xi;Lau, Eric H. Y.;Leung, Gabriel M.
通讯作者：
Leung, Gabriel M.