Kinesin-5 mediated chromosome congression in insect spindles.
Kinesin-5 mediated chromosome congression in insect spindles.
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DOI：
10.1007/s12195-017-0500-0
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发表时间：
2018-03
影响因子：
2.8
通讯作者：
Odde DJ
中科院分区：
文献类型：
--
作者：
Tubman E;He Y;Hays TS;Odde DJ
The microtubule motor protein kinesin-5 is well known to establish the bipolar spindle by outward sliding of antiparallel interpolar microtubules. In yeast, kinesin-5 also facilitates chromosome alignment “congression” at the spindle equator by preferentially depolymerizing long kinetochore microtubules (kMTs). The motor protein kinesin-8 has also been linked to chromosome congression. Therefore, we sought to determine whether kinesin-5 or kinesin-8 facilitates chromosome congression in insect spindles. RNAi of the kinesin-5 Klp61F and kinesin-8 Klp67A were performed separately in Drosophila melanogaster S2 cells to test for inhibited chromosome congression. Klp61F RNAi, Klp67A RNAi, and control metaphase mitotic spindles expressing fluorescent tubulin and fluorescent Cid were imaged, and their fluorescence distributions were compared. RNAi of Klp61F with a weak Klp61F knockdown resulted in longer kMTs and less congressed kinetochores compared to control over a range of conditions, consistent with kinesin-5 length-dependent depolymerase activity. RNAi of the kinesin-8 Klp67A revealed that kMTs relative to the spindle lengths were not longer compared to control, but rather that the spindles were longer, indicating that Klp67A acts preferentially as a length-dependent depolymerase on interpolar microtubules without significantly affecting kMT length and chromosome congression. This study demonstrates that in addition to establishing the bipolar spindle, kinesin-5 regulates kMT length to facilitate chromosome congression in insect spindles. It expands on previous yeast studies, and it expands the role of kinesin-5 to include kMT assembly regulation in eukaryotic mitosis.
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DOI：
10.1016/j.cub.2009.04.036
发表时间：
2009-05-26
期刊：
Current biology : CB
影响因子：
--
作者：
Ke K;Cheng J;Hunt AJ
通讯作者：
Hunt AJ
影响因子：
3.3
作者：
McCoy KM;Tubman ES;Claas A;Tank D;Clancy SA;O'Toole ET;Berman J;Odde DJ
通讯作者：
Odde DJ
DOI：
10.1083/jcb.118.1.109
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoyt MA;He L;Loo KK;Saunders WS
通讯作者：
Saunders WS
影响因子：
9.2
作者：
Goshima, G;Wollman, R;Vale, RD
通讯作者：
Vale, RD
DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
Clemens, JC;Worby, CA;Dixon, JE
通讯作者：
Dixon, JE