RNA versatility, flexibility, and thermostability for practice in RNA nanotechnology and biomedical applications.
RNA versatility, flexibility, and thermostability for practice in RNA nanotechnology and biomedical applications.
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DOI：
10.1002/wrna.1452
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Guo P
中科院分区：
文献类型：
--
作者：
Haque F;Pi F;Zhao Z;Gu S;Hu H;Yu H;Guo P
近年来，RNA作为一种独特的生物材料，具有独特的生物物理特性，可以在纳米尺度上设计复杂的结构，引起了人们的广泛关注。RNA在结构和功能上比其核酸对应物DNA更通用，具有更高的热力学稳定性。较大的RNA分子可以被视为由通过不同接头序列连接的许多“乐高”构建块的组合构建的模块化结构。通过利用RNA基序的多样性和结构的灵活性，可以精确控制形状，大小和化学计量来制造各种RNA架构。多年来已经发现和表征了许多结构基序，并且这些基序中的许多基序的晶体结构可用于纳米颗粒构建。例如，利用RNA结构的灵活性和多功能性，RNA三角形、正方形、五边形和六边形可以由phi 29 pRNA-三向连接（3 WJ）结构单元构建。本次审查将集中在二维RNA三角形，正方形和六聚体; 3D和4D结构建立的基本RNA积木;和他们的前瞻性应用在体内成像或治疗剂通过特定的交付和靶向。细胞内克隆和表达的RNA分子和RNA纳米粒子的体内组装的方法也将进行审查。来源于噬菌体phi 29 DNA包装马达的包装RNA的3 WJ基序是高度生物稳定的。3 WJ可以被调整以构建RNA三角形和正方形。通过分子间的相互作用可以构建RNA六聚体。RNA三角形单元可以进一步组装成RNA二维三角形、正方形、五聚体、六聚体和阵列以及三维结构，包括四面体、棱柱和树枝状聚合物。多功能RNA纳米颗粒作为肿瘤靶向治疗的载体显示出巨大的潜力。
In recent years, RNA has attracted widespread attention as a unique biomaterial with distinct biophysical properties for designing sophisticated architectures in the nanometer scale. RNA is much more versatile in structure and function with higher thermodynamic stability compared to its nucleic acid counterpart DNA. Larger RNA molecules can be viewed as a modular structure built from a combination of many ‘Lego’ building blocks connected via different linker sequences. By exploiting the diversity of RNA motifs and flexibility of structure, varieties of RNA architectures can be fabricated with precise control of shape, size, and stoichiometry. Many structural motifs have been discovered and characterized over the years and the crystal structures of many of these motifs are available for nanoparticle construction. For example, using the flexibility and versatility of RNA structure, RNA triangles, squares, pentagons and hexagons can be constructed from phi29 pRNA-three-way junction (3WJ) building block. This review will focus on 2D RNA triangles, squares and hexamers; 3D and 4D structures built from basic RNA building blocks; and their prospective applications in vivo as imaging or therapeutic agents via specific delivery and targeting. Methods for intracellular cloning and expression of RNA molecules and the in vivo assembly of RNA nanoparticles will also be reviewed. The 3WJ motif derived from the packaging RNA of bacteriophage phi29 DNA packaging motor is highly thermodynamically stable. The 3WJ can be tuned to construct RNA triangles and squares. Through intermolecular interaction RNA hexamer can be constructed. The RNA triangular units can be further assembled into RNA 2D triangle, square, pentamer, hexamer, and arrays as well as 3D structures including tetrahedron, prism and dendrimers. The multi-functional RNA nanoparticles have shown enormous potential as delivery vehicles for targeted cancer therapy.
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影响因子：
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作者：
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