Modulation of gene expression in heart and liver of hibernating black bears (Ursus americanus).
Modulation of gene expression in heart and liver of hibernating black bears (Ursus americanus).
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DOI：
10.1186/1471-2164-12-171
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发表时间：
2011-03-31
期刊：
影响因子：
4.4
通讯作者：
Barnes BM
中科院分区：
文献类型：
--
作者：
Fedorov VB;Goropashnaya AV;Tøien Ø;Stewart NC;Chang C;Wang H;Yan J;Showe LC;Showe MK;Barnes BM
冬眠是一种适应性策略，以在高度季节性或不可预测的环境中生存。哺乳动物冬眠生理学的分子和遗传基础最近才被大规模的基因组学方法研究。我们分析了基因表达在美国黑熊，Ursus americanus，使用自定义12,800 cDNA探针微阵列检测表达的差异，发生在心脏和肝脏在冬季冬眠期间相比，夏季活跃的动物。我们确定了245个基因在心脏和319个基因在肝脏之间的差异表达的冬季和夏季。24个基因在冬眠期间心脏和肝脏的表达显著升高。这些基因主要参与脂质代谢和蛋白质生物合成，包括RNA结合蛋白基序3（Rbm 3），其在轻度低温下增强蛋白质合成。蛋白质生物合成基因的高表达表明，诱导翻译可能与适应机制有关，在长时间的低代谢和冬眠期间的不动性，减少心脏和肌肉萎缩。参与氨基酸分解代谢的基因转录的协同减少表明氨基酸从分解代谢途径重定向到蛋白质生物合成。我们确定了黑熊和小型哺乳动物冬眠动物肝脏中常见的转录变化，包括诱导负责脂肪酸β氧化和碳水化合物合成的基因，以及抑制参与脂质生物合成、碳水化合物催化、细胞呼吸和解毒途径的基因。我们的研究结果表明，在冬季冬眠的基因表达的调制代表了适应极端环境的分子机制。
Hibernation is an adaptive strategy to survive in highly seasonal or unpredictable environments. The molecular and genetic basis of hibernation physiology in mammals has only recently been studied using large scale genomic approaches. We analyzed gene expression in the American black bear, Ursus americanus, using a custom 12,800 cDNA probe microarray to detect differences in expression that occur in heart and liver during winter hibernation in comparison to summer active animals. We identified 245 genes in heart and 319 genes in liver that were differentially expressed between winter and summer. The expression of 24 genes was significantly elevated during hibernation in both heart and liver. These genes are mostly involved in lipid catabolism and protein biosynthesis and include RNA binding protein motif 3 (Rbm3), which enhances protein synthesis at mildly hypothermic temperatures. Elevated expression of protein biosynthesis genes suggests induction of translation that may be related to adaptive mechanisms reducing cardiac and muscle atrophies over extended periods of low metabolism and immobility during hibernation in bears. Coordinated reduction of transcription of genes involved in amino acid catabolism suggests redirection of amino acids from catabolic pathways to protein biosynthesis. We identify common for black bears and small mammalian hibernators transcriptional changes in the liver that include induction of genes responsible for fatty acid β oxidation and carbohydrate synthesis and depression of genes involved in lipid biosynthesis, carbohydrate catabolism, cellular respiration and detoxification pathways. Our findings show that modulation of gene expression during winter hibernation represents molecular mechanism of adaptation to extreme environments.
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影响因子：
4.6
作者：
Brauch, KM;Dhruv, ND;Andrews, MT
通讯作者：
Andrews, MT
影响因子：
1.4
作者：
Barboza, PS;Farley, SD;Robbins, CT
通讯作者：
Robbins, CT
影响因子：
7
作者：
Carninci, P;Shibata, Y;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
4.6
作者：
Fedorov, Vadim B.;Goropashnaya, Anna V.;Barnes, Brian M.
通讯作者：
Barnes, Brian M.
皮肤T细胞淋巴瘤白血病阶段患者的生存分类和预测。
DOI：
10.1084/jem.20021726
发表时间：
2003-06-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kari L;Loboda A;Nebozhyn M;Rook AH;Vonderheid EC;Nichols C;Virok D;Chang C;Horng WH;Johnston J;Wysocka M;Showe MK;Showe LC
通讯作者：
Showe LC