Direct quantification of cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry.
Direct quantification of cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry.
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DOI：
10.1007/s00216-011-5197-7
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发表时间：
2011-09
影响因子：
4.3
通讯作者：
Huestis, Marilyn A.
中科院分区：
文献类型：
--
作者：
Schwope, David M.;Scheidweiler, Karl B.;Huestis, Marilyn A.
建立了液相色谱-串联质谱（LC-MS/MS）测定全血中大麻素和大麻素葡萄糖醛酸酯的方法。固相萃取后，乙腈沉淀蛋白质。HPLC梯度洗脱，分离时间16 min。电喷雾电离法检测大麻素；采用阳性极性（Δ9-tetrahydrocannabinol [THC]、大麻酚[CBN]）和阴性极性（11-羟基-THC [11-OH-THC]、11-对-9-羧基-THC [THCCOOH]、大麻二酚[CBD]、THC-葡糖苷和THCCOOH -葡糖苷）进行多反应监测。线性回归分析采用氘标记的内标，加权系数为1/x2，所有分析物的R2值为> 0.997。线性范围为0.5 ~ 50 μg/L （THC-glucuronide）、1.0 ~ 100 μg/L （THC、11-OH-THC、THCCOOH、CBD和CBN）和5.0 ~ 250 μg/L （thccoh -glucuronide）。在线性范围内，三种浓度下，所有分析物的不精密度< 10.5% CV，回收率为50.5%，偏差在目标的±13.1%以内。未观察到结转、内源性或外源性干扰。这种新的分析方法将有助于全血中大麻素的定量，并进一步研究大麻素葡萄糖醛酸盐作为近期大麻摄入量的标记物。
The first method for quantifying cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. Solid-phase extraction followed protein precipitation with acetonitrile. HPLC separation was achieved in 16 min via gradient elution. Electrospray ionization was utilized for cannabinoid detection; both positive (Δ9-tetrahydrocannabinol [THC], cannabinol [CBN]) and negative (11-hydroxy-THC [11-OH-THC], 11-nor-9-carboxy-THC [THCCOOH], cannabidiol [CBD], THC-glucuronide and THCCOOH glucuronide) polarity were employed with multiple reaction monitoring. Calibration by linear regression analysis utilized deuterium-labeled internal standards and a 1/x2 weighting factor, yielding R2 values > 0.997 for all analytes. Linearity ranged from 0.5–50 μg/L (THC-glucuronide), 1.0–100 μg/L (THC, 11-OH-THC, THCCOOH, CBD and CBN) and 5.0–250 μg/L (THCCOOH-glucuronide). Imprecision was < 10.5% CV, recovery was > 50.5% and bias within ± 13.1% of target for all analytes at three concentrations across the linear range. No carryover, endogenous or exogenous interferences were observed. This new analytical method should be useful for quantifying cannabinoids in whole blood and further investigating cannabinoid glucuronides as markers of recent cannabis intake.
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影响因子：
2.5
作者：
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通讯作者：
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作者：
Schwilke, Eugene W.;Schwope, David M.;Huestis, Marilyn A.
通讯作者：
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