Genome-Wide Identification and Expression Analysis of the Histone Deacetylase Gene Family in Wheat (Triticum aestivum L.).
Genome-Wide Identification and Expression Analysis of the Histone Deacetylase Gene Family in Wheat (Triticum aestivum L.).
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DOI：
10.3390/plants10010019
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发表时间：
2020-12-24
期刊：
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Jin P;Gao S;He L;Xu M;Zhang T;Zhang F;Jiang Y;Liu T;Yang J;Yang J;Dai L;Chen J
组蛋白乙酰化是由组蛋白乙酰转移酶（HAT）和组蛋白去乙酰化酶（HDAC）共同调控的动态修饰过程。尽管HDACs在非生物或生物胁迫应答中发挥重要作用，但其在小麦中的成员及其对植物病毒的应答尚不清楚。在这里，我们鉴定并表征了49 T。小麦HDAC（TaHDAC）的全基因组水平。系统发育分析表明，TaHDACs可分为5个进化枝，其蛋白质空间结构完整且保守。染色体定位和同线性分析表明，TaHDAC基因广泛分布于小麦染色体上，基因重复加速了TaHDAC基因家族的进化。顺式作用元件分析表明，TaHDACs参与激素响应、光响应、非生物胁迫、生长发育等过程。RNA测序数据的热图分析表明，TaHDAC基因参与生物或非生物胁迫反应。所选TaHDAC在不同组织中或在不同温度条件下差异表达。所有选择的TaHDAC与大麦条纹花叶病毒（BSMV），中国小麦花叶病毒（CWMV），小麦黄花叶病毒（WYMV）感染后显着上调，表明它们参与响应病毒感染。此外，TaSRT 1沉默有助于提高小麦对CWMV感染的抗性。这些研究结果有助于加深对小麦HDAC基因家族结构和特性的理解，并为探索TaHDAC在植物抗病毒感染中的功能奠定基础。
Histone acetylation is a dynamic modification process co-regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Although HDACs play vital roles in abiotic or biotic stress responses, their members in Triticum aestivum and their response to plant viruses remain unknown. Here, we identified and characterized 49 T. aestivum HDACs (TaHDACs) at the whole-genome level. Based on phylogenetic analyses, TaHDACs could be divided into 5 clades, and their protein spatial structure was integral and conserved. Chromosomal location and synteny analyses showed that TaHDACs were widely distributed on wheat chromosomes, and gene duplication has accelerated the TaHDAC gene family evolution. The cis-acting element analysis indicated that TaHDACs were involved in hormone response, light response, abiotic stress, growth, and development. Heatmaps analysis of RNA-sequencing data showed that TaHDAC genes were involved in biotic or abiotic stress response. Selected TaHDACs were differentially expressed in diverse tissues or under varying temperature conditions. All selected TaHDACs were significantly upregulated following infection with the barley stripe mosaic virus (BSMV), Chinese wheat mosaic virus (CWMV), and wheat yellow mosaic virus (WYMV), suggesting their involvement in response to viral infections. Furthermore, TaSRT1-silenced contributed to increasing wheat resistance against CWMV infection. In summary, these findings could help deepen the understanding of the structure and characteristics of the HDAC gene family in wheat and lay the foundation for exploring the function of TaHDACs in plants resistant to viral infections.
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