Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids.
Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids.
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DOI：
10.1126/sciadv.abj2800
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发表时间：
2021-08
期刊：
影响因子：
13.6
通讯作者：
Perelman LT
中科院分区：
文献类型：
--
作者：
Pettinato G;Coughlan MF;Zhang X;Chen L;Khan U;Glyavina M;Sheil CJ;Upputuri PK;Zakharov YN;Vitkin E;D'Assoro AB;Fisher RA;Itzkan I;Zhang L;Qiu L;Perelman LT
可移植可再生的hiPSC类器官的形成可以通过实时无标签细胞内光谱来辅助。由人类诱导多能干细胞（hiPSCs）形成的类器官可能是用于许多器官移植的功能组织的无限来源。不幸的是，以可控、可扩展和可复制的方式对分化方案进行微调以形成大量的hiPSC类器官是相当困难的，并且通常需要很长时间。最近，我们介绍了一种利用微制造细胞排斥微孔阵列从分离的hipsc和内皮细胞快速形成类器官的新方法。这种方法，当与实时无标记的生化成分变化拉曼光谱和染色质转变的共聚焦光散射光谱显微镜相结合时，允许在不牺牲样品的情况下监测活体分化类器官，大大缩短了方案微调的时间。我们使用这种方法来培养和监测具有人类肝脏主要功能特征的同质肝类器官，这些器官可用于人类细胞移植肝治疗。
Formation of transplantable reproducible hiPSC organoids can be aided with real-time label-free intracellular spectroscopy. Organoids formed from human induced pluripotent stem cells (hiPSCs) could be a limitless source of functional tissue for transplantations in many organs. Unfortunately, fine-tuning differentiation protocols to form large quantities of hiPSC organoids in a controlled, scalable, and reproducible manner is quite difficult and often takes a very long time. Recently, we introduced a new approach of rapid organoid formation from dissociated hiPSCs and endothelial cells using microfabricated cell-repellent microwell arrays. This approach, when combined with real-time label-free Raman spectroscopy of biochemical composition changes and confocal light scattering spectroscopic microscopy of chromatin transition, allows for monitoring live differentiating organoids without the need to sacrifice a sample, substantially shortening the time of protocol fine-tuning. We used this approach to both culture and monitor homogeneous liver organoids that have the main functional features of the human liver and which could be used for cell transplantation liver therapy in humans.
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影响因子：
3.7
作者：
HASMY, A;FORET, M;JULLIEN, R
通讯作者：
JULLIEN, R
影响因子：
64.8
作者：
Dixon JR;Jung I;Selvaraj S;Shen Y;Antosiewicz-Bourget JE;Lee AY;Ye Z;Kim A;Rajagopal N;Xie W;Diao Y;Liang J;Zhao H;Lobanenkov VV;Ecker JR;Thomson JA;Ren B
通讯作者：
Ren B
DOI：
10.1126/science.1256271
发表时间：
2014-08-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lara-Astiaso D;Weiner A;Lorenzo-Vivas E;Zaretsky I;Jaitin DA;David E;Keren-Shaul H;Mildner A;Winter D;Jung S;Friedman N;Amit I
通讯作者：
Amit I
影响因子：
8.8
作者：
Ang LT;Tan AKY;Autio MI;Goh SH;Choo SH;Lee KL;Tan J;Pan B;Lee JJH;Lum JJ;Lim CYY;Yeo IKX;Wong CJY;Liu M;Oh JLL;Chia CPL;Loh CH;Chen A;Chen Q;Weissman IL;Loh KM;Lim B
通讯作者：
Lim B
影响因子：
4.8
作者：
Behbahan, Iman Saramipoor;Duan, Yuyou;Lam, Alexander;Khoobyari, Shiva;Ma, Xiaocui;Ahuja, Tijess P.;Zern, Mark A.
通讯作者：
Zern, Mark A.