Imaging the developing heart: synchronized time-lapse microscopy during developmental changes

Imaging the developing heart: synchronized time-lapse microscopy during developmental changes
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发育中的心脏成像:发育变化过程中的同步延时显微镜

DOI:
10.1117/12.2290191
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发表时间:
2018
期刊:
--
影响因子:
--
通讯作者:
Taylor J
Taylor J
中科院分区:
--
文献类型:
--
作者:
Taylor J

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你如何使用成像来分析心脏的发育,它不仅改变形状,而且还经历恒定,高速,准周期性的变化?我们整合了前瞻性和回顾性光学门控的想法,以捕获长期的锁相发育延时视频。在本文中,我们证明了这种方法在关键发育时间段的成功:心脏循环,其中心脏形状的巨大变化阻止了之前的前瞻性门控方法捕获锁相视频。我们使用与其他方法在体内心脏成像的比较,以突出收集最合适的数据的生物学问题的重要性。
How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.
DOI: 10.1038/nmeth.3037
发表时间: 2014-09-01
期刊: NATURE METHODS
影响因子: 48
作者:
Mickoleit, Michaela;Schmid, Benjamin;Huisken, Jan
通讯作者: Huisken, Jan
DOI: --
发表时间: 2006
期刊: IEEE International Symposium on Biomedical Imaging
影响因子: --
作者:
M. Liebling;J. Vermot;A. Forouhar;M. Gharib;M. Dickinson;S. Fraser
通讯作者: S. Fraser