Accumulation and deposition of triacylglycerols in the starchy endosperm of wheat grain.
Accumulation and deposition of triacylglycerols in the starchy endosperm of wheat grain.
复制标题
小麦籽粒淀粉胚乳中三酰甘油的积累与沉积。
DOI：
10.1016/j.jcs.2021.103167
复制
发表时间：
2021-03
影响因子：
3.8
通讯作者：
Shewry PR
中科院分区：
文献类型：
--
作者：
González-Thuillier I;Pellny TK;Tosi P;Mitchell RAC;Haslam R;Shewry PR
结合脂质组学、转录组学和生物成像技术，研究了小麦淀粉胚乳发育过程中三酰甘油的合成和沉积。TAG含量在开花后14至34天之间增加，在两个实验中从50至115 mg/100 g干重和从约35至175 mg/100 g干重。在整个发育过程中，主要脂肪酸为C16（棕榈酸C16：0和棕榈油酸C16：1）和C18（硬脂酸C18：0、油酸C18：1、亚油酸C18：2和亚麻酸C18：3），不饱和脂肪酸约占总量的75-80%。亚油酸（C18：2）在各发育阶段均为主要成分，且随发育比例增加。转录谱分析表明TAG合成和油积累的主要途径是通过肯尼迪途径和二酰基甘油酰基转移酶（DGAT）活性。染色组织切片的共聚焦显微镜显示，TAG积累在与蛋白质相关的液滴中，并集中在糊粉层下的淀粉胚乳细胞中。还表达了编码16 kd油质蛋白的转录物，表明油滴部分地被油质蛋白稳定。小麦胚乳中三酰甘油合成与沉积的综合研究。小麦淀粉胚乳中三酰甘油组成的首次脂质组学分析。淀粉胚乳细胞脂质沉积的生物成像。转录组分析显示肯尼迪途径用于脂质合成的操作。
A combination of lipidomics, transcriptomics and bioimaging has been used to study triacylglycerol synthesis and deposition in the developing starchy endosperm of wheat. The content of TAG increased between 14 and 34 days after anthesis, from 50 to 115 mg/100 g dry wt and from about 35 to 175 mg/100 g dry wt in two experiments. The major fatty acids were C16 (palmitic C16:0 and palmitoleic C16:1) and C18 (stearic C18:0, oleic C18:1, linoleic C18:2 and linolenic C18:3), with unsaturated fatty acids accounting for about 75–80% of the total throughout development. Linoleic acid (C18:2) was the major component at all stages and the proportion increased during development. Transcript profiling indicated that predominant route to TAG synthesis and oil accumulation is via the Kennedy pathway and diacylglycerol acyltransferase (DGAT) activity. Confocal microscopy of stained tissue sections showed that TAG accumulated in droplets which are associated with protein and concentrated in the starchy endosperm cells below the sub-aleurone cells. Transcripts encoding 16kd oleosins were also expressed, indicating that the oil droplets are in part stabilised by oleosin proteins. Integrated study of triacylglycerol synthesis and deposition in wheat endosperm. First lipidomic analysis of triacylglycerol composition in wheat starchy endosperm. Bioimaging of lipid deposition in starchy endosperm cells. Transcriptome analysis shows operation of Kennedy pathway for lipid synthesis.
登录
查看更多内容
影响因子：
10.7
作者：
Salt LJ;González-Thuillier I;Chope G;Penson S;Tosi P;Haslam RP;Skeggs PK;Shewry PR;Wilde PJ
通讯作者：
Wilde PJ
DOI：
10.3390/molecules23102583
发表时间：
2018-10-09
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
de Carvalho CCCR;Caramujo MJ
通讯作者：
Caramujo MJ
DOI：
10.1199/tab.0133
发表时间：
2010-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Li-Beisson, Yonghua;Shorrosh, Basil;Ohlrogge, John
通讯作者：
Ohlrogge, John
影响因子：
7.4
作者：
Pellny, Till K.;Lovegrove, Alison;Mitchell, Rowan A. C.
通讯作者：
Mitchell, Rowan A. C.
使用同量异位串联质量标签标记对人工老化和引发过程中小麦种子进行定量蛋白质组分析
影响因子：
3.7
作者：
Lv Y;Zhang S;Wang J;Hu Y
通讯作者：
Hu Y