Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling.
Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling.
复制标题
使用同量异位串联质量标签标记对人工老化和引发过程中小麦种子进行定量蛋白质组分析
DOI：
10.1371/journal.pone.0162851
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hu Y
中科院分区：
文献类型：
--
作者：
Lv Y;Zhang S;Wang J;Hu Y
小麦（Triticum aestivum L.）是世界范围内的重要作物。种子在贮藏和引发期间的生理劣化与发芽密切相关，因此有助于植物生长和随后的谷物产量。在这项研究中，小麦种子在人工老化（45°C; 50%相对湿度; 98%，50%，20%，和1%发芽率）和引发（水引发处理）的不同阶段进行蛋白质组学分析，通过蛋白质组学方法的基础上的同量异位串联质量标签标记。在人工衰老过程中共鉴定了162个差异表达蛋白（DEPs），主要涉及代谢、能量供应和防御/应激反应，从而验证了先前的生理生化研究。这些DEPs表明，不能防止老化导致储存物质的逐步分解，代谢和能量供应受损，最终导致种子劣变。京都基因与基因组百科全书（KEGG）分析表明，参与种子老化的上调蛋白主要富集在核糖体中，而下调蛋白主要积累在能量供应（淀粉和蔗糖代谢）和胁迫防御（抗坏血酸和乙醛酸代谢）中。蛋白质，包括血红蛋白1，油质蛋白，凝集素，和非特异性脂质转移蛋白，首次在老化种子中被确定，并可能被视为种子劣变的新标志。在所鉴定的蛋白质中，与未引发的种子相比，在种子引发期间识别了531个DEP。与种子老化过程中上调的DEPs相反，引发过程中上调的DEPs参与能量供应（三羧酸循环、糖酵解和脂肪酸氧化）、同化（氨基酸和脂肪酸合成）和细胞生长/分裂。KEGG和蛋白质互作分析表明，在种子引发过程中表达上调的蛋白质主要集中在氨基酸合成、逆境防御（植物-病原菌互作、抗坏血酸和醛糖二酸代谢）和能量供应（氧化磷酸化和碳代谢）等方面。因此，与种子老化和引发相关的DEP可用于表征种子活力和优化发芽促进处理。这项工作揭示了新的蛋白质组学的见解，发生在种子变质和引发蛋白质的变化。
Wheat (Triticum aestivum L.) is an important crop worldwide. The physiological deterioration of seeds during storage and seed priming is closely associated with germination, and thus contributes to plant growth and subsequent grain yields. In this study, wheat seeds during different stages of artificial ageing (45°C; 50% relative humidity; 98%, 50%, 20%, and 1% Germination rates) and priming (hydro-priming treatment) were subjected to proteomics analysis through a proteomic approach based on the isobaric tandem mass tag labeling. A total of 162 differentially expressed proteins (DEPs) mainly involved in metabolism, energy supply, and defense/stress responses, were identified during artificial ageing and thus validated previous physiological and biochemical studies. These DEPs indicated that the inability to protect against ageing leads to the incremental decomposition of the stored substance, impairment of metabolism and energy supply, and ultimately resulted in seed deterioration. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the up-regulated proteins involved in seed ageing were mainly enriched in ribosome, whereas the down-regulated proteins were mainly accumulated in energy supply (starch and sucrose metabolism) and stress defense (ascorbate and aldarate metabolism). Proteins, including hemoglobin 1, oleosin, agglutinin, and non-specific lipid-transfer proteins, were first identified in aged seeds and might be regarded as new markers of seed deterioration. Of the identified proteins, 531 DEPs were recognized during seed priming compared with unprimed seeds. In contrast to the up-regulated DEPs in seed ageing, several up-regulated DEPs in priming were involved in energy supply (tricarboxylic acid cycle, glycolysis, and fatty acid oxidation), anabolism (amino acids, and fatty acid synthesis), and cell growth/division. KEGG and protein-protein interaction analysis indicated that the up-regulated proteins in seed priming were mainly enriched in amino acid synthesis, stress defense (plant-pathogen interactions, and ascorbate and aldarate metabolism), and energy supply (oxidative phosphorylation and carbon metabolism). Therefore, DEPs associated with seed ageing and priming can be used to characterize seed vigor and optimize germination enhancement treatments. This work reveals new proteomic insights into protein changes that occur during seed deterioration and priming.
登录
查看更多内容
影响因子：
64.8
作者：
通讯作者：
--
中国小麦优良品种小偃6号籽粒发育过程中白蛋白和球蛋白动态
影响因子：
3.8
作者：
Dong, Kun;Ge, Pei;Yan, Yueming
通讯作者：
Yan, Yueming
DOI：
10.1007/978-1-61779-231-1_20
发表时间：
2011-01-01
期刊：
SEED DORMANCY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Bailly, Christophe;Kranner, Ilse
通讯作者：
Kranner, Ilse
影响因子：
3
作者：
Cao, Bihao;Lu, Yaqing;Lei, Jianjun
通讯作者：
Lei, Jianjun
影响因子：
7.4
作者：
Gallardo, K;Job, C;Job, D
通讯作者：
Job, D