Effects of Ozone Treatment on Personal Protective Equipment Contaminated with SARS-CoV-2.
Effects of Ozone Treatment on Personal Protective Equipment Contaminated with SARS-CoV-2.
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DOI：
10.3390/antiox9121222
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发表时间：
2020-12-03
期刊：
影响因子：
--
通讯作者：
Lorenzo-Morales J
中科院分区：
文献类型：
--
作者：
Clavo B;Córdoba-Lanús E;Rodríguez-Esparragón F;Cazorla-Rivero SE;García-Pérez O;Piñero JE;Villar J;Blanco A;Torres-Ascensión C;Martín-Barrasa JL;González-Martin JM;Serrano-Aguilar P;Lorenzo-Morales J
背景：严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 正在全球范围内造成严重的健康、经济和社会问题。个人防护装备 (PPE) 的管理及其潜在的有限供应引发了人们对医院和疗养院医疗保健专业人员面临的风险增加的担忧。臭氧是一种强氧化剂。本研究的目的是检查臭氧处理对受 SARS-CoV-2 污染的个人防护装备的影响，并探讨相对湿度是否可以改变这些影响。方法：用不同的臭氧浓度、暴露时间和相对湿度条件处理被热灭活的 SARS-CoV-2 污染的个人防护装备。通过实时聚合酶链式反应评估 SARS-CoV-2 基因扩增。结果：在以下臭氧暴露后，个人防护装备中的 SARS-CoV-2 没有扩增：10,000 ppm (20 g/m3) 下 30 秒、4000 ppm 下 5 分钟和 2000 ppm 下 10 分钟。在较低的臭氧浓度（4–12 ppm（0.008–0.024 g/m3））下，其影响高度依赖于相对湿度条件。结论：在适当的暴露时间、臭氧浓度和相对湿度条件下，臭氧暴露引起的氧化应激消除了不同 PPE 成分中热灭活的 SARS-CoV-2。这些发现可能对降低与个人防护设备管理相关的污染风险并提高其可用性产生影响。保证了对原始 SARS-CoV-2 毒株的进一步研究。
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing profound health, economic, and social problems worldwide. Management of personal protective equipment (PPE) and its potential limited availability have created concerns about the increased risks for healthcare professionals at hospitals and nursing homes. Ozone is a powerful oxidant agent. The objectives of this study were to examine the effects of ozone treatment on PPE contaminated with SARS-CoV-2, and to explore whether relative humidity could modify those effects. Methods: PPE contaminated by heat-inactivated SARS-CoV-2 were treated with different ozone concentrations, exposure times, and relative humidity conditions. SARS-CoV-2 gene amplification was assessed by real-time polymerase chain reaction. Results: There was no amplification of SARS-CoV-2 in PPE after the following ozone exposures: 30 s at 10,000 ppm (20 g/m3), 5 min at 4000 ppm, and 10 min at 2000 ppm. At lower ozone concentrations, 4–12 ppm (0.008–0.024 g/m3), the effects were highly dependent on the relative humidity conditions. Conclusions: Oxidative stress induced by ozone exposure eliminated heat-inactivated SARS-CoV-2 in different PPE components under appropriate exposure times, ozone concentrations, and relative humidity conditions. These findings could have implications in decreasing the risk of contamination associated with personal protective equipment management and in increasing its availability. Further research in the original SARS-CoV-2 strain is guaranteed.
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