Release and reloading of intracellular Ca stores after cholinergic stimulation of the parietal cell.
Release and reloading of intracellular Ca stores after cholinergic stimulation of the parietal cell.
复制标题
壁细胞胆碱能刺激后细胞内 Ca 储存的释放和重新加载。
DOI：
10.1152/ajpcell.1988.254.4.c498
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Machen,TE
中科院分区：
文献类型：
--
作者：
Negulescu,PA;Machen,TE
使用显微荧光分光光度法测量负载 Ca 敏感性荧光染料 fura-2 的完整兔胃腺的单个壁细胞中的胞质游离 Ca，Cai。用胆碱能激动剂卡巴胆碱反复刺激细胞，以深入了解激素刺激的钙代谢所涉及的膜机制。在含 Ca 或不含 Ca 的溶液中，卡巴胆碱 (100 µM) 会导致 Cai 从静止水平 100 nM 快速（在 30 秒内）升高至大于 600 nM。峰值后，Cai 在 3 分钟内下降至较基线稍高（大于 200 nM）的较低水平。该平台依赖于卡巴胆碱和细胞外 Ca (Cao)，并且可以通过添加阿托品 (1 µM) 或镧离子 (La, 50 µM) 来阻断。尖峰是由于 Ca 从内部储存释放，而平台期是由于 Ca 通过激动剂控制的、La 抑制的通道进入质膜。在卡巴胆碱刺激 3 分钟或更长时间后，内部存储的重新加载完全取决于 Cao。在这些条件下，重新加载通过质膜中的 La 敏感（但硝苯地平和维拉帕米不敏感）途径发生。在重新加载过程中，没有检测到蔡的显着变化。与较长的治疗相反，如果用阿托品终止卡巴胆碱刺激，同时 Cai 仍升高，则细胞质会发生显着的重新加载。（摘要截断为 250 字）
Microspectrofluorimetry was used to measure cytosolic free Ca, Cai, in single parietal cells of intact rabbit gastric glands loaded with the Ca-sensitive fluorescent dye, fura-2. Cells were repeatedly stimulated with the cholinergic agonist carbachol to gain insights into the membrane mechanisms involved in hormonally stimulated Ca metabolism. In either Ca-containing or Ca-free solutions, carbachol (100 microM) caused a rapid (within 30 s) elevation of Cai from a resting level of 100 nM to greater than 600 nM. After the spike, Cai decreased within 3 min to a lower level that was somewhat elevated (greater than 200 nM) over base line. This plateau was dependent on both carbachol and extracellular Ca (Cao) and could be blocked by the addition of atropine (1 microM) or lanthanum ion (La, 50 microM). The spike is due to the release of Ca from internal stores, whereas the plateau is due to Ca entry across the plasma membrane through agonist-controlled, La-inhibitable channels. After a carbachol stimulation of 3 min or longer, reloading of the internal store was absolutely dependent on Cao. Under these conditions, reloading occurred through a La-sensitive (but nifedipine- and verapamil-insensitive) pathway in the plasma membrane. No significant change in Cai was detectable during the reloading. In contrast to the longer treatments, if carbachol stimulation was terminated with atropine while Cai was still elevated, significant reloading occurred from the cytosol.(ABSTRACT TRUNCATED AT 250 WORDS)
人 T 淋巴细胞质膜中肌醇 1,4,5-三磷酸激活的离子通道
影响因子：
64.8
作者：
M. Kuno;P. Gardner
通讯作者：
P. Gardner
DOI：
10.1111/j.1748-1716.1976.tb10184.x
发表时间：
1976-01-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
BERGLINDH, T;OBRINK, KJ
通讯作者：
OBRINK, KJ
影响因子：
4
作者：
TSIEN, RY;RINK, TJ;POENIE, M
通讯作者：
POENIE, M