Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean (Glycine max L.).
Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean (Glycine max L.).
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大豆 ERF 基因家族的系统发育、基因结构和表达模式 (Glycine max L.)
DOI：
10.1093/jxb/ern248
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发表时间：
2008
影响因子：
6.9
通讯作者：
Ma, Youzhi
中科院分区：
文献类型：
--
作者：
Zhang, Gaiyun;Chen, Ming;Chen, Xueping;Xu, Zhaoshi;Guan, Shan;Li, Lian-Cheng;Li, Aili;Guo, Jiaming;Mao, Long;Ma, Youzhi
ERF转录因子家族的成员在调节基因表达以应对生物和非生物胁迫方面发挥着重要作用。然而，在大豆 (Glycine max L.) 中，迄今为止仅研究了少数 ERF 基因。在这项研究中，从 DFCI 大豆基因索引数据库中的 63-676 个独特序列中鉴定出 98 个包含完整 AP2/ERF 结构域的 unigene。分析了大豆 ERF 蛋白的系统发育、基因结构和推定的保守基序，并与拟南芥和水稻进行了比较。大豆 ERF 家族的成员被分为 12 个亚群，与拟南芥的情况类似。 AP2/ERF 结构域在大豆、拟南芥和水稻中是保守的。在 AP2/ERF 结构域之外，检测到许多大豆特有的保守基序。表达分析表明，属于六个 ERF 家族亚群的 9 个 unigene 受到生物/非生物胁迫和激素处理的诱导，表明它们参与生物和非生物胁迫响应信号通路之间的串扰。来自两个不同亚群的两个全长基因的过度表达增强了烟草植物对干旱、盐胁迫和/或病原体感染的耐受性。这些结果将有助于阐明大豆中 ERF 基因相关的应激反应信号通路。
Members of the ERF transcription factor family play important roles in regulating gene expression in response to biotic and abiotic stresses. In soybean (Glycine max L.), however, only a few ERF genes have been studied so far. In this study, 98 unigenes that contained a complete AP2/ERF domain were identified from 63 676 unique sequences in the DFCI Soybean Gene Index database. The phylogeny, gene structures, and putative conserved motifs in soybean ERF proteins were analysed, and compared with those of Arabidopsis and rice. The members of the soybean ERF family were divided into 12 subgroups, similar to the case for Arabidopsis. AP2/ERF domains were conserved among soybean, Arabidopsis, and rice. Outside the AP2/ERF domain, many soybean-specific conserved motifs were detected. Expression analysis showed that nine unigenes belonging to six ERF family subgroups were induced by both biotic/abiotic stresses and hormone treatment, suggesting that they were involved in cross-talk between biotic and abiotic stress-responsive signalling pathways. Overexpression of two full-length genes from two different subgroups enhanced the tolerances to drought, salt stresses, and/or pathogen infection of the tobacco plants. These results will be useful for elucidating ERF gene-associated stress response signalling pathways in soybean.
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影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.2
作者：
Kizis, D;Pagès, M
通讯作者：
Pagès, M
影响因子：
44.1
作者：
Hu, YX;Wang, YH;Li, JY
通讯作者：
Li, JY
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
7.2
作者：
Berrocal-Lobo, M;Molina, A;Solano, R
通讯作者：
Solano, R