Interspecific germline transmission of cultured primordial germ cells.
Interspecific germline transmission of cultured primordial germ cells.
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DOI：
10.1371/journal.pone.0035664
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Etches RJ
中科院分区：
文献类型：
--
作者：
van de Lavoir MC;Collarini EJ;Leighton PA;Fesler J;Lu DR;Harriman WD;Thiyagasundaram TS;Etches RJ
在鸟类中，原始生殖细胞(PGC)谱系在受精后24小时内从胞体分离。在这里，我们展示了来自单个鸡胚的大约200个PGC的内源性种群可以在培养中扩大100万倍。当培养的PGCs被注射到处于相同发育阶段的异种胚胎中时，它们会在睾丸中定植。在性成熟时，这些供体PGCs在异种宿主中进行精子生成，成为有功能的精子。将异种宿主的精液授精给供体物种的雌性，产生供体物种的正常后代。在我们的模型系统中，供体物种是鸡，受体物种是珍珠鸡(Numida Meleagris)，这表明控制种系增殖的机制在鸟类中是高度保守的。从实用的角度来看，这些数据是一种新战略的基础，该战略利用驯化的宿主生产濒危物种的鸟类，这些宿主既容易驯化，又多产。
In birds, the primordial germ cell (PGC) lineage separates from the soma within 24 h following fertilization. Here we show that the endogenous population of about 200 PGCs from a single chicken embryo can be expanded one million fold in culture. When cultured PGCs are injected into a xenogeneic embryo at an equivalent stage of development, they colonize the testis. At sexual maturity, these donor PGCs undergo spermatogenesis in the xenogeneic host and become functional sperm. Insemination of semen from the xenogeneic host into females from the donor species produces normal offspring from the donor species. In our model system, the donor species is chicken (Gallus domesticus) and the recipient species is guinea fowl (Numida meleagris), a member of a different avian family, suggesting that the mechanisms controlling proliferation of the germline are highly conserved within birds. From a pragmatic perspective, these data are the basis of a novel strategy to produce endangered species of birds using domesticated hosts that are both tractable and fecund.
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影响因子：
3.7
作者：
Macdonald J;Glover JD;Taylor L;Sang HM;McGrew MJ
通讯作者：
McGrew MJ
影响因子：
2.5
作者：
Leighton, Philip A.;Van De Lavoir, Marie-Cecile;Etches, Robert J.
通讯作者：
Etches, Robert J.
影响因子：
64.8
作者：
van de lavoir, Marie-Cecile;Diamond, Jennifer H.;Etches, Robert J.
通讯作者：
Etches, Robert J.
DOI：
10.1186/1297-9686-41-9
发表时间：
2009-01-14
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Oliehoek PA;Bijma P
通讯作者：
Bijma P
影响因子：
1.5
作者：
Li, Hai-Chang;Zhao, Li;Ono, Tamao
通讯作者：
Ono, Tamao