High mRNA Expression of CENPL and Its Significance in Prognosis of Hepatocellular Carcinoma Patients.
High mRNA Expression of CENPL and Its Significance in Prognosis of Hepatocellular Carcinoma Patients.
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DOI：
10.1155/2021/9971799
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Cui Z;Xiao L;Chen F;Wang J;Lin H;Li D;Wu Z
着丝粒蛋白（CENPs）是着丝点的主要组成蛋白，是细胞分裂所必需的。近年来，一些研究发现几种CENPs在肝细胞癌（HCC）中异常表达。然而，许多着丝粒蛋白尚未在HCC中得到研究。在这项研究中，我们使用Oncomine、基因表达谱交互分析（GEPIA）、Kaplan-Meier绘图仪、cBioPortal、人类蛋白质图谱（HPA）和TIMER（肿瘤免疫估计资源）数据库和临床标本的免疫组织化学染色来研究HCC中15种主要着中心点蛋白的表达，以评估其潜在的预后价值。我们发现15种着丝粒蛋白中的4种（CENPL、CENPQ、CENPR和CENPU） mRNA水平在HCC中显著高于正常组织，并且它们的mRNA水平与肿瘤分期相关（p值< 0.01）。CENPL mRNA水平较高的患者总生存期、无进展生存期、无复发生存期和疾病特异性生存期较差（p值< 0.05）。此外，在没有肝炎病毒感染的男性中，较高水平的CENPL mRNA与较差的总生存率相关（p值< 0.05）。人HCC组织中CENPL蛋白表达水平高于正常肝组织。此外，CENPL的表达与肿瘤浸润淋巴细胞水平呈正相关。结果提示，高表达的CENPL mRNA可能是HCC患者预后的潜在预测因子。
Centromere proteins (CENPs) are the main constituent proteins of kinetochore, which are essential for cell division. In recent years, several studies have revealed that several CENPs were aberrantly expressed in hepatocellular carcinoma (HCC). However, numerous centromere proteins have not been studied in HCC. In this study, we used databases of Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), the Kaplan-Meier Plotter, cBioPortal, the Human Protein Atlas (HPA), and TIMER (Tumor Immune Estimation Resource) and immunohistochemical staining of clinical specimens to investigate the expression of 15 major centromere proteins in HCC to evaluate their potential prognostic value. We found that the mRNA levels of 4 out of 15 centromere proteins (CENPL, CENPQ, CENPR, and CENPU) were significantly higher in HCC than in normal tissues, and their mRNA levels were associated with the tumor stages (p values < 0.01). Patients with higher mRNA levels of CENPL had poorer overall survival, progression-free survival, relapse-free survival, and disease-specific survival (p values < 0.05). Furthermore, the higher levels of CENPL mRNA were associated with worse overall survival in males without hepatitis virus infection (p values < 0.05). The protein expression level of CENPL in human HCC tissue was higher than that in normal liver tissue. In addition, the expression of CENPL was positively correlated with the levels of the tumor-infiltrating lymphocytes. The results suggest that the high mRNA expression of CENPL may be a potential predictor of prognosis in HCC patients.
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DOI：
10.1038/nrm2718
发表时间：
2009-07
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
GEPIA：用于癌症和正常基因表达谱和交互式分析的网络服务器。
影响因子：
14.9
作者：
Tang Z;Li C;Kang B;Gao G;Li C;Zhang Z
通讯作者：
Zhang Z
DOI：
10.18632/aging.101441
发表时间：
2018-05-11
期刊：
Aging
影响因子：
--
作者：
Sun CC;Zhou Q;Hu W;Li SJ;Zhang F;Chen ZL;Li G;Bi ZY;Bi YY;Gong FY;Bo T;Yuan ZP;Hu WD;Zhan BT;Zhang Q;Tang QZ;Li DJ
通讯作者：
Li DJ
影响因子：
4.2
作者：
Lu, Guifang;Shan, Tao;Zhang, Dan
通讯作者：
Zhang, Dan
影响因子：
3.3
作者：
Chen, X;Cheung, ST;Brown, PO
通讯作者：
Brown, PO