Phorbol esters induce angiogenesis in vitro from large‐vessel endothelial cells
Phorbol esters induce angiogenesis in vitro from large‐vessel endothelial cells
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佛波酯在体外诱导大血管内皮细胞血管生成
DOI：
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发表时间：
1987
影响因子：
5.6
通讯作者：
L. Orci
中科院分区：
文献类型：
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作者：
R. Montesano;L. Orci
我们之前已经证明，肿瘤启动子phorbol肉豆酸酯（PMA）诱导生长在三维胶原凝胶表面的毛细血管内皮细胞侵入底层基质，形成毛细血管样管状结构，这种现象模拟了体内发生的血管生成过程（Montesano and Orci: Cell 42:469, 1985）。为了确定毛细血管样芽侵入细胞外基质的潜力是否仅限于微血管内皮细胞，还是也与大血管内皮细胞共享，我们研究了从人脐静脉和小牛肺动脉分离的内皮细胞对PMA的反应。实验结果表明，经PMA处理后，两种类型的大血管内皮细胞都能够像血管样管道一样渗透到胶原凝胶中。这表明来自大血管的内皮细胞可以响应适当的信号，表达被认为与体内毛细血管内皮细胞特异性相关的侵袭性特性。
We have shown previously that the tumor promoter phorbol myristate acetate (PMA) induces capillary endothelial cells grown on the surface of threedimensional collagen gels to invade the underlying matrix as capillary‐like tubular structures, a phenomenon mimicking angiogenic processes that occur in vivo (Montesano and Orci: Cell 42:469, 1985). To establish whether the potential to invade the extracellular matrix as capillary‐like sprouts is restricted to microvascular endothelial cells or is also shared by large vessel endothelium, we have examined the response to PMA of endothelial cells isolated from the human umbilical vein and the calf pulmonary artery. The results of these experiments show that both types of macrovascular endothelial cells are able to penetrate into collagen gels as vessel‐like tubes following treatment with PMA. This demonstrates that endothelial cells derived from large vessels can, in response to appropriate signals, express invasive properties thought to be associated specifically with capillary endothelial cells in vivo.
人脐静脉内皮细胞响应血管生成制剂产生潜在胶原酶。
影响因子：
3.7
作者：
Moscatelli,DA;Rifkin,DB;Jaffe,EA
通讯作者：
Jaffe,EA
影响因子：
56.9
作者：
THORNTON, SC;MUELLER, SN;LEVINE, EM
通讯作者：
LEVINE, EM