Silencing of the glycerophosphocholine phosphodiesterase GDPD5 alters the phospholipid metabolite profile in a breast cancer model in vivo as monitored by (31) P MRS.
Silencing of the glycerophosphocholine phosphodiesterase GDPD5 alters the phospholipid metabolite profile in a breast cancer model in vivo as monitored by (31) P MRS.
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DOI：
10.1002/nbm.3106
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发表时间：
2014-06
影响因子：
2.9
通讯作者：
Glunde, K.
中科院分区：
文献类型：
--
作者：
Wijnen, J. P.;Jiang, L.;Greenwood, T. R.;Cheng, M.;Doepkens, M.;Cao, M. D.;Bhujwalla, Z. M.;Krishnamachary, B.;Klomp, D. W. J.;Glunde, K.
胆碱磷脂代谢异常是一种新出现的癌症标志，其与癌症发生和肿瘤进展有关。恶性代谢表型的特征在于侵袭性乳腺癌细胞中高水平的磷酸胆碱（PC）和相对低水平的甘油磷酸胆碱（GPC）。磷磁共振波谱（31 P MRS）能够无创地检测胆碱的这些水溶性代谢物以及乙醇胺磷脂代谢。在这里，我们研究了稳定沉默甘油磷酸酯二酯酶域5（GDPD 5），这是一种酶与甘油磷酸胆碱磷酸二酯酶活性，在MDA-MB-231乳腺癌细胞和原位肿瘤异种移植物的影响。与对照肿瘤相比，其中GDPD 5被GDPD 5特异性shRNA稳定沉默的肿瘤含有增加的GPC和磷酸乙醇胺（PE）水平。
Abnormal choline phospholipid metabolism is an emerging hallmark of cancer, which is implicated in carcinogenesis and tumor progression. The malignant metabolic phenotype is characterized by high levels of phosphocholine (PC) and relatively low levels of glycerophosphocholine (GPC) in aggressive breast cancer cells. Phosphorus Magnetic Resonance Spectroscopy (31P MRS) is able to noninvasively detect these water-soluble metabolites of choline as well as ethanolamine phospholipid metabolism. Here we have investigated the effects of stably silencing glycerophosphoester diesterase domain containing 5 (GDPD5), which is an enzyme with glycerophosphocholine phosphodiesterase activity, in MDA-MB-231 breast cancer cells and orthotopic tumor xenografts. Tumors in which GDPD5 was stably silenced with GDPD5-specific shRNA contained increased levels of GPC and phosphoethanolamine (PE) compared to control tumors.
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影响因子：
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作者：
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通讯作者：
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