Metabolism of 2-chloro-4-nitrophenol in a gram negative bacterium, Burkholderia sp. RKJ 800.
Metabolism of 2-chloro-4-nitrophenol in a gram negative bacterium, Burkholderia sp. RKJ 800.
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DOI：
10.1371/journal.pone.0038676
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jain RK
中科院分区：
文献类型：
--
作者：
Arora PK;Jain RK
采用富集法从印度某农药污染场地分离到一株2-氯-4-硝基苯酚（2C 4 NP）降解菌RKJ 800，该菌以2C 4 NP为唯一碳源和能源。在2C 4 NP的降解过程中检测到化学计量的亚硝酸根和氯离子。通过薄层色谱、高效液相色谱和气相色谱-质谱联用技术，确定了2C 4 NP降解途径中的主要代谢产物为氯代对苯二酚（CHQ）和对苯二酚（HQ）。在2C 4 NP诱导的菌株RKJ 800的细胞的粗提物中观察到锰依赖性HQ双加氧酶活性，这表明HQ裂解为γ-羟基粘康酸半醛。经16 S rRNA基因序列测定，确定RKJ 800为伯克霍尔德氏菌属（Burkholderia）。我们的研究清楚地表明，伯克霍尔德氏菌RKJ 800通过对苯二酚途径降解2-氯-4-硝基苯酚。在革兰氏阴性菌Burkholderia sp.菌株RKJ 800中鉴定的途径与先前报道的另一种革兰氏阴性Burkholderia sp. SJ 98中的2C 4 NP降解途径不同。这是第一次报道任何革兰氏阴性菌降解2C 4 NP时形成CHQ和HQ。实验室规模的土壤微宇宙研究表明，菌株RKJ 800是一个合适的候选人2C 4 NP污染的网站的生物修复。
A 2-Chloro-4-nitrophenol (2C4NP) degrading bacterial strain designated as RKJ 800 was isolated from a pesticide contaminated site of India by enrichment method and utilized 2C4NP as sole source of carbon and energy. The stoichiometric amounts of nitrite and chloride ions were detected during the degradation of 2C4NP. On the basis of thin layer chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry, chlorohydroquinone (CHQ) and hydroquinone (HQ) were identified as major metabolites of the degradation pathway of 2C4NP. Manganese dependent HQ dioxygenase activity was observed in the crude extract of 2C4NP induced cells of the strain RKJ 800 that suggested the cleavage of the HQ to γ-hydroxymuconic semialdehyde. On the basis of the 16S rRNA gene sequencing, strain RKJ 800 was identified as a member of genus Burkholderia. Our studies clearly showed that Burkholderia sp. RKJ 800 degraded 2-chloro-4-nitrophenol via hydroquinone pathway. The pathway identified in a gram negative bacterium, Burkholderia sp. strain RKJ 800 was differed from previously reported 2C4NP degradation pathway in another gram-negative Burkholderia sp. SJ98. This is the first report of the formation of CHQ and HQ in the degradation of 2C4NP by any gram-negative bacteria. Laboratory-scale soil microcosm studies showed that strain RKJ 800 is a suitable candidate for bioremediation of 2C4NP contaminated sites.
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影响因子：
3.6
作者：
Arora, Pankaj Kumar;Jain, Rakesh Kumar
通讯作者：
Jain, Rakesh Kumar
影响因子：
2.8
作者：
BRUHN, C;BAYLY, RC;KNACKMUSS, HJ
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DOI：
10.1099/ijs.0.023184-0
发表时间：
2011-08-01
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作者：
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