N-acetylmannosamine (ManNAc) supports the growth of Borrelia burgdorferi in the absence of N-acetylglucosamine (GlcNAc)
N-acetylmannosamine (ManNAc) supports the growth of Borrelia burgdorferi in the absence of N-acetylglucosamine (GlcNAc)
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N-乙酰甘露糖胺 (ManNAc) 在缺乏 N-乙酰氨基葡萄糖 (GlcNAc) 的情况下支持伯氏疏螺旋体的生长
DOI：
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发表时间：
2018
影响因子：
2.1
通讯作者：
Ryan G. Rhodes
中科院分区：
文献类型：
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作者：
E. Schneider;Ryan G. Rhodes
莱姆病的病原体伯氏疏螺旋体缺乏从头生物合成许多必需营养素的能力，包括N-乙酰葡糖胺（GlcNAc）。这种氨基糖是细胞壁合成所需的，并且是用于体外繁殖的复杂生长培养基的组分。当在没有游离GlcNAc的情况下培养时，B.伯氏细胞表现出独特的双相生长模式。我们假设与完全培养基中培养的细胞相比，参与GlcNAc饥饿反应的基因将差异表达，并使用转录组学对此进行了研究。与用GlcNAc培养的野生型细胞相比，在无GlcNAc培养的野生型和饥饿适应细胞中有21个基因差异调节。其中，参与碳水化合物利用的三个基因被上调：bbb 04（chbC）编码壳二糖转运蛋白的亚基，bb 0629（fruA-2）编码推定的碳水化合物转运蛋白，bb 0644（nanE）编码推定的GlcNAc-6-磷酸-2-差向异构酶，预计可催化N-乙酰甘露糖胺-6-磷酸的转化使用定量RT-PCR来确认选择基因的差异表达，并且用游离ManNAc取代游离GlcNAc导致生长至高细胞密度，表明B。burgdorferi细胞可以利用游离ManNAc进行细胞壁合成和能量产生。
Borrelia burgdorferi, the causative agent of Lyme disease, lacks the ability to biosynthesize many essential nutrients de novo, including N-acetylglucosamine (GlcNAc). This amino sugar is required for cell wall synthesis, and is a component of the complex growth medium used for in vitro propagation. When cultured without free GlcNAc, B. burgdorferi cells exhibit a unique biphasic growth pattern. We hypothesized that genes involved in the GlcNAc starvation response would be differentially expressed when compared to cells cultured in complete medium, and investigated this using transcriptomics. Twenty-one genes were differentially regulated in wild-type and starvation-adapted cells cultured without GlcNAc compared to wild-type cells cultured with GlcNAc. Of those, three genes involved in carbohydrate utilization were upregulated: bbb04 (chbC) encoding a subunit of the chitobiose transporter, bb0629 (fruA-2) encoding a putative carbohydrate transporter and bb0644 (nanE) encoding a putative GlcNAc-6-phosphate-2-epimerase predicted to catalyze the conversion of N-acetylmannosamine-6-phosphate (ManNAc-6-P) to GlcNAc-6-P. Quantitative RT-PCR was used to confirm differential expression of select genes, and substitution of free GlcNAc with free ManNAc resulted in growth to high cell density, suggesting B. burgdorferi cells can utilize free ManNAc for cell wall synthesis and energy production.
影响因子：
3.7
作者：
Haines-Menges BL;Whitaker WB;Lubin JB;Boyd EF
通讯作者：
Boyd EF
影响因子：
3.7
作者：
Corona A;Schwartz I
通讯作者：
Schwartz I