In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-alpha and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1.
In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-alpha and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1.
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DOI：
10.3164/jcbn.08-182
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发表时间：
2009-01
影响因子：
2.4
通讯作者：
Döring F
中科院分区：
文献类型：
--
作者：
Schmelzer C;Lorenz G;Rimbach G;Döring F
泛醇-10（QH 2）是辅酶Q10（CoQ 10）的还原形式，可作为脂质膜的有效抗氧化剂。由于许多抗氧化剂显示出有效的抗炎作用，因此在人单核细胞系THP-1中测定了QH 2对脂多糖（LPS）诱导的促炎细胞因子和趋化因子的影响。用LPS刺激细胞导致肿瘤坏死因子-α（TNF-α）、巨噬细胞炎性蛋白-1 α（MIP-1α）、活化调节的正常T细胞表达和分泌（RANTES）和单核细胞趋化蛋白-1（MCP-1）的明显释放。通过用QH 2预孵育细胞，TNF-α、MIP-1α和RANTES的LPS诱导反应分别显著降低至60.27 ± 9.3%（p = 0.0009）、48.13 ± 6.93%（p = 0.0007）和74.36 ± 7.25%（p = 0.008）。总之，我们的研究结果表明辅酶Q10的还原形式在体外对各种促炎细胞因子和趋化因子的抗炎作用。
Ubiquinol-10 (QH2), the reduced form of Coenzyme Q10 (CoQ10) serves as a potent antioxidant of lipid membranes. Because many antioxidants reveal potent anti-inflammatory effects, the influence of QH2 on lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and chemokines were determined in the human monocytic cell line THP-1. Stimulation of cells with LPS resulted in a distinct release of Tumour necrosis factor-alpha (TNF-α), Macrophage inflammatory protein-1 alpha (MIP-1α), Regulated upon activation, normal T cell expressed and secreted (RANTES) and Monocyte chemotattractant protein-1 (MCP-1). The LPS-induced responses were significantly decreased by pre-incubation of cells with QH2 to 60.27 ± 9.3% (p = 0.0009), 48.13 ± 6.93% (p = 0.0007) and 74.36 ± 7.25% (p = 0.008) for TNF-α, MIP-1α and RANTES, respectively. In conclusion, our results indicate anti-inflammatory effects of the reduced form of CoQ10 on various proinflammatory cytokines and chemokines in vitro.
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影响因子：
7.4
作者：
Lee, CK;Pugh, TD;Prolla, TA
通讯作者：
Prolla, TA
DOI：
10.1152/ajplung.00329.2003
发表时间：
2004-06-01
影响因子：
4.9
作者：
Antonicelli, F;Brown, D;MacNee, W
通讯作者：
MacNee, W
影响因子：
2.4
作者：
Kettawan, Aikkarach;Takahashi, Takayuki;Okamoto, Tadashi
通讯作者：
Okamoto, Tadashi
影响因子：
4.8
作者：
Sanlioglu, S;Williams, CM;Engelhardt, JF
通讯作者：
Engelhardt, JF
影响因子：
19
作者：
Schreck, R;Baeuerle, P A
通讯作者：
Baeuerle, P A