Yeast endocytic adaptor AP-2 binds the stress sensor Mid2 and functions in polarized cell responses.
Yeast endocytic adaptor AP-2 binds the stress sensor Mid2 and functions in polarized cell responses.
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DOI：
10.1111/tra.12155
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Ayscough KR
中科院分区：
文献类型：
--
作者：
Chapa-y-Lazo B;Allwood EG;Smaczynska-de Rooij II;Snape ML;Ayscough KR
AP-2复合物是异源四聚体内吞货物结合衔接子，其在哺乳动物网格蛋白介导的内吞作用期间促进膜蛋白的摄取。虽然芽殖酵母具有所有四个AP-2亚基的明确同源物，其形成复合物并定位于体内的内吞位点，但酵母AP-2的功能仍然是谜。在这里，我们证明，AP-2是所需的菌丝生长在白色念珠菌和极化细胞反应在酿酒酵母。AP-2的货物结合mu亚基APM 4的缺失导致假菌丝生长、交配突起的产生和细胞壁损伤反应的缺陷。在apm 4无效突变体中，细胞壁应力传感器Mid 2无法重新定位到交配突起的尖端，或者响应于细胞壁损伤而重新定位到母芽颈部。Mid 2和Apm 4的mu同源结构域之间的直接结合相互作用进一步支持AP-2结合Mid 2以促进其响应于特定信号的内化和再定位的模型。因此，Mid 2是在酵母中鉴定的AP-2的第一个货物。我们提出，Mid 2和其他成分的内吞再循环是极化细胞反应所必需的，确保细胞壁沉积，并在细胞生长过程中受到严密监测。
The AP-2 complex is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during mammalian clathrin-mediated endocytosis. While budding yeast has clear homologues of all four AP-2 subunits which form a complex and localize to endocytic sites in vivo, the function of yeast AP-2 has remained enigmatic. Here, we demonstrate that AP-2 is required for hyphal growth in Candida albicans and polarized cell responses in Saccharomyces cerevisiae. Deletion of APM4, the cargo-binding mu subunit of AP-2, causes defects in pseudohyphal growth, generation of a mating projection and the cell wall damage response. In an apm4 null mutant, the cell wall stress sensor Mid2 is unable to relocalize to the tip of a mating projection following pheromone addition, or to the mother bud neck in response to cell wall damage. A direct binding interaction between Mid2 and the mu homology domain of Apm4 further supports a model in which AP-2 binds Mid2 to facilitate its internalization and relocalization in response to specific signals. Thus, Mid2 is the first cargo for AP-2 identified in yeast. We propose that endocytic recycling of Mid2 and other components is required for polarized cell responses ensuring cell wall deposition and is tightly monitored during cell growth.
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DOI：
10.1083/jcb.201206081
发表时间：
2013-02-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jose M;Tollis S;Nair D;Sibarita JB;McCusker D
通讯作者：
McCusker D
影响因子：
4
作者：
Krause, Sue Ann;Cundell, Michael J.;Gray, Joseph V.
通讯作者：
Gray, Joseph V.
DOI：
10.1083/jcb.135.2.341
发表时间：
1996-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Marks MS;Woodruff L;Ohno H;Bonifacino JS
通讯作者：
Bonifacino JS
影响因子：
64.8
作者：
Kelly, Bernard T.;McCoy, Airlie J.;Spaete, Kira;Miller, Sharon E.;Evans, Philip R.;Hoening, Stefan;Owen, David J.
通讯作者：
Owen, David J.
影响因子：
16
作者：
Nern, A;Arkowitz, RA
通讯作者：
Arkowitz, RA