Genomewide transcriptional signatures of migratory flight activity in a globally invasive insect pest.
Genomewide transcriptional signatures of migratory flight activity in a globally invasive insect pest.
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DOI：
10.1111/mec.13362
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发表时间：
2015-10
影响因子：
4.9
通讯作者：
Chapman JW
中科院分区：
文献类型：
--
作者：
Jones CM;Papanicolaou A;Mironidis GK;Vontas J;Yang Y;Lim KS;Oakeshott JG;Bass C;Chapman JW
迁徙是许多动物生活史上的一个关键策略，需要一系列行为、形态和生理适应，这些适应共同形成了“迁徙综合征”。遗传变异已被证明是构成这种综合征的许多特征，但涉及的潜在基因仍然难以捉摸。最近研究迁移相关基因的研究集中在从不同地理位置采样迁移和非迁移种群，但很少探索迁移性状的表型变异。在这里，我们使用一种新的拴系飞行和下一代测序的组合来确定与全球入侵蛾害虫棉铃虫（Helicoverpa armigera）飞行活动相关的转录组差异。通过开发一个最先进的表型分析平台，我们发现野外采集的棉铃虫在飞行性能上表现出连续的变化，个体能够在一个晚上飞行40公里。飞行表型的比较转录组学推动了基因表达分析，揭示了一系列表达的候选基因，这些基因与长途飞行所需的生理适应明显相关。这些基因包括对作为飞行燃料的脂质的动员、飞行肌肉结构的发育和影响迁徙生理的激素的调节至关重要的基因。我们的结论是，能够表达这一套复杂的途径强调了显着的灵活性兼性昆虫移民应对日益恶化的条件下的形式迁移飞行，更广泛地说，结果提供了新的见解的基本转录变化所需的迁移昆虫和其他类群。
Migration is a key life history strategy for many animals and requires a suite of behavioural, morphological and physiological adaptations which together form the ‘migratory syndrome’. Genetic variation has been demonstrated for many traits that make up this syndrome, but the underlying genes involved remain elusive. Recent studies investigating migration‐associated genes have focussed on sampling migratory and nonmigratory populations from different geographic locations but have seldom explored phenotypic variation in a migratory trait. Here, we use a novel combination of tethered flight and next‐generation sequencing to determine transcriptomic differences associated with flight activity in a globally invasive moth pest, the cotton bollworm Helicoverpa armigera. By developing a state‐of‐the‐art phenotyping platform, we show that field‐collected H. armigera display continuous variation in flight performance with individuals capable of flying up to 40 km during a single night. Comparative transcriptomics of flight phenotypes drove a gene expression analysis to reveal a suite of expressed candidate genes which are clearly related to physiological adaptations required for long‐distance flight. These include genes important to the mobilization of lipids as flight fuel, the development of flight muscle structure and the regulation of hormones that influence migratory physiology. We conclude that the ability to express this complex set of pathways underlines the remarkable flexibility of facultative insect migrants to respond to deteriorating conditions in the form of migratory flight and, more broadly, the results provide novel insights into the fundamental transcriptional changes required for migration in insects and other taxa.
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DOI：
10.1016/s0305-0491(97)00185-5
发表时间：
1997-08-01
影响因子：
2.2
作者：
Haunerland, NH
通讯作者：
Haunerland, NH
影响因子：
1.5
作者：
COLVIN, J;GATEHOUSE, AG
通讯作者：
GATEHOUSE, AG
DOI：
10.1016/s0305-0491(97)00212-5
发表时间：
1997-08-01
影响因子：
2.2
作者：
Candy, DJ;Becker, A;Wegener, G
通讯作者：
Wegener, G
新型储存产品 Psocid、Liposcelis badia 的温度依赖性发育和繁殖（Psocoptera：Liposcelididae）
影响因子：
1.7
作者：
Feng, Hong-Qiang;Zhao, Xin-Cheng;Guo, Yu-Yuan
通讯作者：
Guo, Yu-Yuan
影响因子：
2.2
作者：
Feng, Hongqiang;Wu, Xianfu;Wu, Kongming
通讯作者：
Wu, Kongming