Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon.
Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon.
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DOI：
10.3389/fpls.2013.00050
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发表时间：
2013
影响因子：
5.6
通讯作者：
Mitchell RA
中科院分区：
文献类型：
--
作者：
Molinari HB;Pellny TK;Freeman J;Shewry PR;Mitchell RA
禾本科植物如小麦、玉米、水稻和甘蔗的细胞壁含有大量的阿魏酸，阿魏酸与细胞壁多糖葡糖醛酸阿拉伯木聚糖（GAX）酯连接。这种阿魏酸酯被认为限制了草生物质中多糖的消化率，因为它在多糖和木质素组分之间形成共价键。BAHD酰基辅酶A转移酶超家族的草特异性分支内的候选基因已被鉴定为负责阿魏酸酯与GAX的酯键。因此，这些BAHD基因的操作可能是提高草生物质转化为生物燃料的效率的生物技术目标。在这里，我们描述了这些候选基因的表达和结合阿魏酸从不同组织和发育阶段的模式草二穗短柄草。BAHD候选转录本和大量的结合阿魏酸存在于每个组织和发育阶段。我们假设BAHD候选基因类似于最近描述的水稻p-香豆酸单甘酯转移酶（OsPMT）基因（PMT亚进化枝）主要负责结合对香豆酸（pCA），和其他BAHD候选人（非PMT亚进化枝）负责结合阿魏酸（FA）。组织类型之间非PMT/PMT基因表达的比率和结合FA/pCA的比率之间存在一些相似性，与该假设一致。然而，需要进一步的工作来修饰禾本科植物中的BAHD基因并表征异源表达的蛋白质以证明它们的功能。
The cell walls of grasses such as wheat, maize, rice, and sugar cane, contain large amounts of ferulate that is ester-linked to the cell wall polysaccharide glucuronoarabinoxylan (GAX). This ferulate is considered to limit the digestibility of polysaccharide in grass biomass as it forms covalent linkages between polysaccharide and lignin components. Candidate genes within a grass-specific clade of the BAHD acyl-coA transferase superfamily have been identified as being responsible for the ester linkage of ferulate to GAX. Manipulation of these BAHD genes may therefore be a biotechnological target for increasing efficiency of conversion of grass biomass into biofuel. Here, we describe the expression of these candidate genes and amounts of bound ferulate from various tissues and developmental stages of the model grass Brachypodium distachyon. BAHD candidate transcripts and significant amounts of bound ferulate were present in every tissue and developmental stage. We hypothesize that BAHD candidate genes similar to the recently described Oryza sativa p-coumarate monolignol transferase (OsPMT) gene (PMT sub-clade) are principally responsible for the bound para-coumaric acid (pCA), and that other BAHD candidates (non-PMT sub-clade) are responsible for bound ferulic acid (FA). There were some similarities with between the ratio of expression non-PMT/PMT genes and the ratio of bound FA/pCA between tissue types, compatible with this hypothesis. However, much further work to modify BAHD genes in grasses and to characterize the heterologously expressed proteins is required to demonstrate their function.
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影响因子：
5.6
作者：
Rancour DM;Marita JM;Hatfield RD
通讯作者：
Hatfield RD
影响因子：
14.9
作者：
Sato Y;Namiki N;Takehisa H;Kamatsuki K;Minami H;Ikawa H;Ohyanagi H;Sugimoto K;Itoh J;Antonio BA;Nagamura Y
通讯作者：
Nagamura Y
影响因子：
4.3
作者：
Piston F;Uauy C;Fu L;Langston J;Labavitch J;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
6.1
作者：
Lu, Fachuang;Wei, Liping;Ralph, John
通讯作者：
Ralph, John
影响因子：
7.4
作者：
Pellny, Till K.;Lovegrove, Alison;Mitchell, Rowan A. C.
通讯作者：
Mitchell, Rowan A. C.