Robust DNA Methylation in the Clonal Raider Ant Brain.
Robust DNA Methylation in the Clonal Raider Ant Brain.
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DOI：
10.1016/j.cub.2015.12.040
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发表时间：
2016-02-08
期刊：
影响因子：
9.2
通讯作者：
Kronauer, Daniel Jan Christoph
中科院分区：
文献类型：
--
作者：
Libbrecht, Romain;Oxley, Peter Robert;Keller, Laurent;Kronauer, Daniel Jan Christoph
群居昆虫由于其巨大的形态和行为可塑性，是表观遗传学的很有前途的模式系统。有报道称，在群居昆虫中，蜂王和工蚁等级之间的DNA甲基化不同，这暗示了DNA甲基化在调节劳动分工方面的作用。为了更好地了解DNA甲基化在群居昆虫中的作用，我们对克隆掠食者蚂蚁Cerapachys biroi的大脑进行了全基因组亚硫酸氢盐测序，这种蚂蚁的群体在繁殖阶段(女王状)和育儿阶段(工蚁状)之间交替。许多胞嘧啶在所有的复制中都被甲基化(在给定的复制中平均有29.5%的胞嘧啶甲基化)，这表明很大比例的比罗氏梭菌脑甲基组是健壮的。强健的DNA甲基化优先发生在与核心功能相关的高表达和稳定表达的基因的外显子CPGS中。我们的分析没有发现蜂王型和工蜂型阶段的DNA甲基化有任何差异，这表明DNA甲基化与比罗氏沼虾繁殖和行为的变化无关。最后，由于生物变异或实验变异，许多胞嘧啶仅在一个样本中甲基化。通过将以前研究中使用的统计方法应用到我们的数据中，我们表明这种样本特定的DNA甲基化可能是先前蜂王和工蚁特定甲基化的结果的基础。我们认为，目前没有证据表明DNA甲基化的全基因组变异与群居昆虫中的女王和工种有关，我们呼吁对现有数据进行更仔细的解释。
Social insects are promising model systems for epigenetics due to their immense morphological and behavioral plasticity. Reports that DNA methylation differs between the queen and worker castes in social insects have implied a role for DNA methylation in regulating division of labor. To better understand the function of DNA methylation in social insects, we performed whole-genome bisulfite sequencing on brains of the clonal raider ant Cerapachys biroi, whose colonies alternate between reproductive (queen-like) and brood care (workerlike) phases. Many cytosines were methylated in all replicates (on average 29.5% of the methylated cytosines in a given replicate), indicating that a large proportion of the C. biroi brain methylome is robust. Robust DNA methylation occurred preferentially in exonic CpGs of highly and stably expressed genes involved in core functions. Our analyses did not detect any differences in DNA methylation between the queen-like and worker-like phases, suggesting that DNA methylation is not associated with changes in reproduction and behavior in C. biroi. Finally, many cytosines were methylated in one sample only, due to either biological or experimental variation. By applying the statistical methods used in previous studies to our data, we show that such sample-specific DNA methylation may underlie the previous findings of queen- and worker-specific methylation. We argue that there is currently no evidence that genome-wide variation in DNA methylation is associated with the queen and worker castes in social insects, and we call for a more careful interpretation of the available data.
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影响因子：
16.8
作者：
Drewell, Robert A.;Lo, Nathan;Oldroyd, Benjamin P.
通讯作者：
Oldroyd, Benjamin P.
影响因子：
2.6
作者：
Hunt, Brendan G.;Glastad, Karl M.;Goodisman, Michael A. D.
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影响因子：
4.9
作者：
Kronauer, Daniel J. C.;Pierce, Naomi E.;Keller, Laurent
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表观遗传学作为达尔文“特殊困难”的答案。
影响因子：
3.7
作者：
Herb BR
通讯作者：
Herb BR
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y