The Escherichia coli ycbQRST operon encodes fimbriae with laminin-binding and epithelial cell adherence properties in Shiga-toxigenic E. coli O157:H7.
The Escherichia coli ycbQRST operon encodes fimbriae with laminin-binding and epithelial cell adherence properties in Shiga-toxigenic E. coli O157:H7.
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大肠杆菌 ycbQRST 操纵子编码产志贺毒素大肠杆菌 O157:H7 中具有层粘连蛋白结合和上皮细胞粘附特性的菌毛。
DOI：
10.1111/j.1462-2920.2009.01906.x
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发表时间：
2009
影响因子：
5.1
通讯作者：
Girón,JorgeA
中科院分区：
文献类型：
--
作者：
Samadder,Partha;Xicohtencatl-Cortes,Juan;Saldaña,Zeus;Jordan,Dianna;Tarr,PhillipI;Kaper,JamesB;Girón,JorgeA
人类致病菌志贺-产毒素大肠杆菌（STEC） O157:H7含有aycbqrstfbrial - like操纵子，该操纵子与在产毒素大肠杆菌中发现的F17菌毛生物发生基因f17adcg家族具有显著的同源性。我们报道了产STEC O157:H7菌株EDL933在37°C的最小Minca培养基中生长，并在粘附上皮细胞的过程中产生了细的氮周菌毛，该菌毛由一个18 kDa的主要亚基组成，其N端氨基酸序列与cbqgene的预测蛋白产物相匹配；与F17a‐A纤维蛋白具有显著的同源性。与F17菌毛类似，纯化的产志在大肠杆菌菌毛和重组YcbQ蛋白融合到His肽标签结合的层粘连蛋白上，但不融合到纤维连接蛋白或胶原蛋白上。因此，我们建议命名为ee。coliYcbQlaminin - binding fimae （ELF）来标记cbqrstoperon编码的fimae。研究了ELF作为产志在大肠杆菌对培养上皮细胞的粘附因子的作用。我们提供了令人信服的证据，证明ELF有助于产志在大肠杆菌在体外对人肠上皮细胞和牛、猪肠道组织的粘附。STEC菌株EDL933中纤维蛋白亚基基因fa （orycbQ）的缺失导致了一个等基因菌株，与亲本菌株相比，该菌株对HEp‐2细胞的粘附率显著降低（60%）。此外，针对纯化ELF的抗体也部分阻断了两种STEC O157:H7菌株的粘附。这些观察结果表明，ELF作为一种辅助粘附因子，与其他已知的冗余粘附素一起，有助于STEC O157:H7的整体粘附特性，为这些生物提供了在不同宿主和环境中生存的生态优势。
The human pathogen Shiga‐toxigenicEscherichia coli(STEC) O157:H7 contains aycbQRSTfimbrial‐like operon, which shares significant homology to the family of F17 fimbrial biogenesis genesf17ADCGfound in enterotoxigenicE. coli. We report that growth of STEC O157:H7 strain EDL933 in minimal Minca medium at 37°C and during adherence to epithelial cells led to the production of fine peritrichous fimbriae, which were found to be composed of a major subunit of 18 kDa whose N‐terminal amino acid sequence matched the predicted protein product of theycbQgene; and showed significant homology to the F17a‐A fimbrin. Similar to the F17 fimbriae, the purified STEC fimbriae and the recombinant YcbQ protein fused to a His peptide tag bound laminin, but not fibronectin or collagen. Thus, we propose the nameE. coliYcbQlaminin‐bindingfimbriae (ELF) to designate the fimbriae encoded by theycbQRSToperon. The role of ELF as an adherence factor of STEC to cultured epithelial cells was investigated. We provide compelling evidence demonstrating that ELF contributes to adherence of STEC to human intestinal epithelial cells and to cow and pig gut tissuein vitro. Deletion in the fimbrin subunit geneelfA(orycbQ) in STEC strain EDL933 led to an isogenic strain, which showed significant reduction (60%) in adherence to HEp‐2 cells in comparison with the parental strain. In addition, antibodies against the purified ELF also partially blocked adherence of two STEC O157:H7 strains. These observations suggest that ELF functions as an accessory adherence factor that, along with other known redundant adhesins, contributes to the overall adhesive properties of STEC O157:H7 providing these organisms with ecological advantages to survive in different hosts and in the environment.
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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影响因子：
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DOI：
10.1016/0360-3016(79)90953-2
发表时间：
1979-01-01
影响因子：
7
作者：
CARLSSON, J;STALNACKE, CG;LARSSON, B
通讯作者：
LARSSON, B