The role of pygopus in the differentiation of intracardiac valves in Drosophila.
The role of pygopus in the differentiation of intracardiac valves in Drosophila.
复制标题
尾肌在果蝇心内瓣膜分化中的作用
DOI：
10.1002/dvg.22724
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发表时间：
2014-01
期刊：
影响因子：
1.5
通讯作者：
Ocorr, Karen
中科院分区：
文献类型：
--
作者：
Tang, Min;Yuan, Wuzhou;Bodmer, Rolf;Wu, Xiushan;Ocorr, Karen
心脏瓣膜起着重要的作用；它们支持单向血液流动，防止血液倒流。Wnt信号在小鼠心脏瓣膜的形成和斑马鱼心脏瓣膜的增殖中起着重要作用，但对其具体信号成分的鉴定尚未得到系统的解决。在参与Wnt信号转导的成分中，首先在果蝇中被确定为Wnt信号转导的核心成分的pygopus （pygo）在瓣膜发育和分化方面尚未被研究。在这里，我们利用果蝇心脏模型来研究pygo在心脏腔室之间瓣膜形成中的作用。我们发现，果蝇心脏特异性pygo基因敲低会导致这些心脏瓣膜区域的扩张，并且它们特有的肌原纤维密集网状结构不会形成，与邻近的心肌细胞相似。相比之下，介导典型Wnt信号转导的转录因子arm/β - Cat、lgs/BCL9或pan/TCF的心脏特异性敲低显示出更弱的瓣膜分化缺陷。与单独的pygo杂合子相比，pygo和Wnt信号组分突变体的双杂合子组合对心脏功能没有额外的影响。这些结果与pygo在成人心室间瓣膜分化中独立于典型Wnt信号的观点一致。创世纪52:19 - 28,2014。©2013 Wiley期刊公司
Cardiac valves serve an important function; they support unidirectional blood flow and prevent blood regurgitation. Wnt signaling plays an important role in the formation of mouse cardiac valves and cardiac valve proliferation in Zebrafish, but identification of the specific signaling components involved has not been addressed systematically. Of the components involved in Wnt signal transduction, pygopus (pygo), first identified as a core component of Wnt signaling in Drosophila, has not yet to be investigated with respect to valve development and differentiation. Here, we take advantage of the Drosophila heart model to study the role of pygo in formation of valves between the cardiac chambers. We found that cardiac‐specific pygo knockdown in the Drosophila heart causes dilation in the region of these cardiac valves, and their characteristic dense mesh of myofibrils does not form and resembles that of neighboring cardiomyocytes. In contrast, heart‐specific knockdown of the transcription factors, arm/β‐Cat, lgs/BCL9, or pan/TCF, which mediates canonical Wnt signal transduction, shows a much weaker valve differentiation defect. Double‐heterozygous combinations of mutants for pygo and the Wnt‐signaling components have no additional effect on heart function compared with pygo heterozygotes alone. These results are consistent with the idea that pygo functions independently of canonical Wnt signaling in the differentiation of the adult interchamber cardiac valves. genesis 52:19–28, 2014. © 2013 Wiley Periodicals, Inc.
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