The effect of spike mutations on SARS-CoV-2 neutralization.
The effect of spike mutations on SARS-CoV-2 neutralization.
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尖峰突变对SARS-CoV-2中和作用的影响
DOI：
10.1016/j.celrep.2021.108890
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发表时间：
2021-03-23
期刊：
影响因子：
8.8
通讯作者：
McCoy LE
中科院分区：
文献类型：
--
作者：
Rees-Spear C;Muir L;Griffith SA;Heaney J;Aldon Y;Snitselaar JL;Thomas P;Graham C;Seow J;Lee N;Rosa A;Roustan C;Houlihan CF;Sanders RW;Gupta RK;Cherepanov P;Stauss HJ;Nastouli E;SAFER Investigators;Doores KJ;van Gils MJ;McCoy LE
多种严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）疫苗显示出保护效果，这很可能是由识别病毒进入蛋白spike的中和抗体介导的。由于新的SARS-CoV-2变种正在迅速出现，如B.1.1.7、B.1.351和P.1谱系，因此了解由原始SARS-CoV-2病毒或当前疫苗感染诱导的抗体应答是否仍然有效至关重要。在这项研究中，我们评估了一系列突变的刺突假型的基础上，从SARS冠状病毒的分歧，然后比较中和的B.1.1.7刺突假型和个别突变。刺突特异性单克隆抗体中和作用显著降低;相比之下，来自2020年初感染个体的多克隆抗体对大多数突变刺突假型仍具有活性，但在少数样本中效力降低。这项工作强调了SARS-CoV-2尖峰的变化可以改变中和敏感性，并强调了对新出现的突变及其对疫苗效力的影响进行有效实时监测的必要性。SARS-CoV-2假型由编码B.1.1.7变异刺突的SARS-CoV-2假型病毒中的氨基酸取代产生氨基酸变化和B.1.1.7可以降低单克隆抗体中和作用对血清的影响最小，仅损失10%对B.1.1.7假型的效力本研究描述了SARS-CoV-2刺突突变体的抗体和恢复期血清的中和作用。Rees-Spear等人显示SARS-CoV氨基酸替换和B.1.1.7变异体可以阻断单克隆抗体中和，并且在轻度疾病后收集的血清样品比在严重疾病后收集的血清样品对尖峰变异的弹性更小。
Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines show protective efficacy, which is most likely mediated by neutralizing antibodies recognizing the viral entry protein, spike. Because new SARS-CoV-2 variants are emerging rapidly, as exemplified by the B.1.1.7, B.1.351, and P.1 lineages, it is critical to understand whether antibody responses induced by infection with the original SARS-CoV-2 virus or current vaccines remain effective. In this study, we evaluate neutralization of a series of mutated spike pseudotypes based on divergence from SARS-CoV and then compare neutralization of the B.1.1.7 spike pseudotype and individual mutations. Spike-specific monoclonal antibody neutralization is reduced dramatically; in contrast, polyclonal antibodies from individuals infected in early 2020 remain active against most mutated spike pseudotypes, but potency is reduced in a minority of samples. This work highlights that changes in SARS-CoV-2 spike can alter neutralization sensitivity and underlines the need for effective real-time monitoring of emerging mutations and their effect on vaccine efficacy. SARS-CoV-2 pseudotypes produced by amino acids substitutions in SARS-CoV SARS-CoV-2 pseudotyped virus that encodes the B.1.1.7 variant spike Amino acid changes and B.1.1.7 can decrease monoclonal antibody neutralization Minimal effect on sera with only 10% losing potency against the B.1.1.7 pseudotype This study describes neutralization by antibodies and convalescent sera of SARS-CoV-2 spike mutants. Rees-Spear et al. show that SARS-CoV amino acid substitutions and the B.1.1.7 variant can block monoclonal antibody neutralization and that serum samples collected following mild illness are less resilient to spike variation than those following severe illness.
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SARS-COV-2血统的估计可传播和影响B.1.1.7在英国。
DOI：
10.1126/science.abg3055
发表时间：
2021-04-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Davies NG;Abbott S;Barnard RC;Jarvis CI;Kucharski AJ;Munday JD;Pearson CAB;Russell TW;Tully DC;Washburne AD;Wenseleers T;Gimma A;Waites W;Wong KLM;van Zandvoort K;Silverman JD;CMMID COVID-19 Working Group;COVID-19 Genomics UK (COG-UK) Consortium;Diaz-Ordaz K;Keogh R;Eggo RM;Funk S;Jit M;Atkins KE;Edmunds WJ
通讯作者：
Edmunds WJ
影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
SARS-COV-2在人类和貂皮之间的貂皮农场上传播，然后回到人类。
DOI：
10.1126/science.abe5901
发表时间：
2021-01-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Oude Munnink BB;Sikkema RS;Nieuwenhuijse DF;Molenaar RJ;Munger E;Molenkamp R;van der Spek A;Tolsma P;Rietveld A;Brouwer M;Bouwmeester-Vincken N;Harders F;Hakze-van der Honing R;Wegdam-Blans MCA;Bouwstra RJ;GeurtsvanKessel C;van der Eijk AA;Velkers FC;Smit LAM;Stegeman A;van der Poel WHM;Koopmans MPG
通讯作者：
Koopmans MPG
影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
影响因子：
4.7
作者：
Gralinski, Lisa E.;Menachery, Vineet D.
通讯作者：
Menachery, Vineet D.