Arginine enhances osteoblastogenesis and inhibits adipogenesis through the regulation of Wnt and NFATc signaling in human mesenchymal stem cells.
Arginine enhances osteoblastogenesis and inhibits adipogenesis through the regulation of Wnt and NFATc signaling in human mesenchymal stem cells.
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DOI：
10.3390/ijms150713010
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发表时间：
2014-07-22
影响因子：
5.6
通讯作者：
Lee JD
中科院分区：
文献类型：
--
作者：
Huh JE;Choi JY;Shin YO;Park DS;Kang JW;Nam D;Choi DY;Lee JD
据报道，精氨酸是一种 α-氨基酸，可发挥有益作用，改善健康问题并防止过度脂肪沉积。在这项研究中，我们研究了精氨酸激活细胞信号传导是否可以诱导人间充质干细胞（MSC）的成骨分化和调节过度的成脂分化。精氨酸以剂量依赖性方式有效诱导 Iα1 型胶原、骨钙素和 ALP 的表达，且不会引起细胞毒性。精氨酸显着增加成骨转录因子 runt 相关转录因子 2 (Runx2)、DIx5 和 osterix 的 mRNA 表达。此外，精氨酸通过减少间充质干细胞中的脂肪细胞形成和甘油三酯（TG）含量以及抑制脂肪形成转录因子过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT/增强子结合蛋白α（C/EBPα）和脂肪酸结合蛋白4（Fabp4）的mRNA表达来证明其抗脂肪形成作用。这种效应与 Wnt5a 和活化 T 细胞核因子 (NFATc) 的表达增加有关，并且被 Wnt 和 NFATc 的拮抗剂消除，这表明 Wnt 和 NFATc 信号在精氨酸诱导的从脂肪生成到成骨细胞生成的转变中发挥作用。总之，这是首次报道精氨酸通过Wnt5a和NFATc信号通路促进成骨和抑制脂肪细胞形成的双重作用。
Arginine, an α-amino acid, has been reported to exert beneficial effects that ameliorate health problems and prevent excessive fat deposition. In this study, we investigated whether the activation of cell signaling by arginine can induce osteogenic differentiation and modulate excessive adipogenic differentiation in human mesenchymal stem cells (MSCs). Arginine potently induced the expression of type Iα1 collagen, osteocalcin, and ALP in a dose-dependent manner without causing cytotoxicity. Arginine significantly increased the mRNA expression of the osteogenic transcription factors runt-related transcription factor 2 (Runx2), DIx5, and osterix. Furthermore, arginine demonstrated its antiadipogenicity by decreasing adipocyte formation and triglyceride (TG) content in MSCs and inhibiting the mRNA expression of the adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), and fatty acid binding protein 4 (Fabp4). This effect was associated with increased expression of Wnt5a, and nuclear factor of activated T-cells (NFATc), and was abrogated by antagonists of Wnt and NFATc, which indicated a role of Wnt and NFATc signaling in the switch from adipogenesis to osteoblastogenesis induced by arginine. In conclusion, this is the first report of the dual action of arginine in promoting osteogenesis and inhibiting adipocyte formation through involving Wnt5a and NFATc signaling pathway.
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影响因子：
2.7
作者：
Hedgepeth, CM;Conrad, LJ;Klein, PS
通讯作者：
Klein, PS
影响因子：
2.7
作者：
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4.9
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64.5
作者：
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通讯作者：
de Crombrugghe, B
影响因子：
4.1
作者：
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