CPK12: A Ca2+-dependent protein kinase balancer in abscisic acid signaling

CPK12: A Ca2+-dependent protein kinase balancer in abscisic acid signaling
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CPK12:脱落酸信号传导中的 Ca2 依赖性蛋白激酶平衡剂

DOI:
10.4161/psb.6.11.17954
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发表时间:
2011-11
影响因子:
2.9
通讯作者:
张大鹏
张大鹏
中科院分区:
生物学4区
文献类型:
--
作者:
张大鹏

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Ca ~(2+)被认为是阿坝信号转导过程中的重要第二信使。Ca 2+依赖性蛋白激酶(CDPKs)是植物中最具代表性的Ca 2+感受器。最近,我们鉴定了拟南芥中的一个CDPK成员CPK 12,它是种子萌发和萌发后生长过程中阿坝信号的负调控因子,这表明CDPK家族的不同成员可能通过在阿坝信号转导中的正、负作用而构成一个调控回路。我们观察到RNA干扰和CPK 12基因的过表达都导致了种子萌发和萌发后生长中ABA超敏感的表型,表明CPK 12介导的阿坝信号通路具有高度的复杂性。CPK 12激活ABA信号负调节子(ABI 2)并磷酸化两个ABA信号正调节子(ABF 1和ABF 4),这可能部分解释了由CPK 12表达的下调和上调诱导的阿坝超敏性。我们的数据表明,CPK 12似乎在拟南芥阿坝信号转导中起平衡器的作用。
Ca2+ is believed to be a critical second messenger in ABA signal transduction. Ca2+-dependent protein kinases (CDPKs) are the best characterized Ca2+ sensors in plants. Recently, we identified an Arabidopsis CDPK member CPK12 as a negative regulator of ABA signaling in seed germination and post-germination growth, which reveals that different members of the CDPK family may constitute a regulation loop by functioning positively and negatively in ABA signal transduction. We observed that both RNA interference and overexpression of CPK12 gene resulted in ABA-hypersensitive phenotypes in seed germination and post-germination growth, suggesting a high complexity of the CPK12-mediated ABA signaling pathway. CPK12 stimulates a negative ABA-signaling regulator (ABI2) and phosphorylates two positive ABA-signaling regulators (ABF1 and ABF4), which may partly explain the ABA hypersensitivity induced by both downregulation and upregulation of CPK12 expression. Our data indicate that CPK12 appears to function as a balancer in ABA signal transduction in Arabidopsis.
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