Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates.
Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates.
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DOI：
10.1111/1751-7915.13712
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发表时间：
2021-11
影响因子：
5.7
通讯作者：
Jiménez JI
中科院分区：
文献类型：
--
作者：
Narancic T;Salvador M;Hughes GM;Beagan N;Abdulmutalib U;Kenny ST;Wu H;Saccomanno M;Um J;O'Connor KE;Jiménez JI
在这篇文章中，我们总结了我们的研究结果，从分析全基因组序列的Pseudomonasumsongensis GO16，我们已经确定了实验的潜力，从PET水解产生的单体的上循环。除了一般的基因组表征外，我们还鉴定并验证了对苯二甲酸酯和乙二醇降解所需的一组基因，以及菌株生产短链和中链聚羟基烷酸酯的能力。 与一次性材料（如聚对苯二甲酸乙二醇酯（PET））相关的一次性文化已经造成了重大的环境问题。将PET废料回收为可生物降解的塑料聚羟基烷酸酯（PHA）为提高资源效率和促进循环经济创造了机会。我们对先前显示将PET衍生的对苯二甲酸（TA）转化为PHA的乌松假单胞菌GO16的基因组进行了测序，并进行了深入的基因组分析。由于存在分解代谢质粒pENK22，GO16可降解除TA外的一系列芳香族底物。通过原儿茶酸降解TA所需的遗传互补被鉴定，并通过将tph操纵子转移到恶臭假单胞菌KT 2440中来确认其功能性，恶臭假单胞菌KT 2440不能自然地利用TA。我们还确定了参与乙二醇（EG）代谢的基因，第二个PET单体，并验证了GO16使用EG作为唯一碳和能量来源的能力。此外，GO16具有用于合成中链和短链长度PHA的基因，并且我们已经证明了该菌株将混合的TA和EG转化为PHA的能力。GO16的代谢多样性突出了这种生物体使用PET废物作为原料进行生物转化的潜力。
In this article we have summarised our findings resulting from analysing the complete genome sequence of Pseudomonas umsongensis GO16 and we have determined experimentally its potential for the upcycling of monomers resulting from PET hydrolysis. Besides a general genomic characterisation, we have identified and validated the set of genes required for terephthalate and ethylene glycol degradation as well as the ability of the strain for producing short and medium‐chain length polyhydroxyalkanoates. The throwaway culture related to the single‐use materials such as polyethylene terephthalate (PET) has created a major environmental concern. Recycling of PET waste into biodegradable plastic polyhydroxyalkanoate (PHA) creates an opportunity to improve resource efficiency and contribute to a circular economy. We sequenced the genome of Pseudomonas umsongensis GO16 previously shown to convert PET‐derived terephthalic acid (TA) into PHA and performed an in‐depth genome analysis. GO16 can degrade a range of aromatic substrates in addition to TA, due to the presence of a catabolic plasmid pENK22. The genetic complement required for the degradation of TA via protocatechuate was identified and its functionality was confirmed by transferring the tph operon into Pseudomonas putida KT2440, which is unable to utilize TA naturally. We also identified the genes involved in ethylene glycol (EG) metabolism, the second PET monomer, and validated the capacity of GO16 to use EG as a sole source of carbon and energy. Moreover, GO16 possesses genes for the synthesis of both medium and short chain length PHA and we have demonstrated the capacity of the strain to convert mixed TA and EG into PHA. The metabolic versatility of GO16 highlights the potential of this organism for biotransformations using PET waste as a feedstock.
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影响因子：
13.6
作者：
Geyer R;Jambeck JR;Law KL
通讯作者：
Law KL
影响因子：
5
作者：
Kenny, Shane T.;Runic, Jasmina Nikodinovic;O'Connor, Kevin E.
通讯作者：
O'Connor, Kevin E.
影响因子：
3
作者：
Ahn, Eunsol;Choi, Ki Young;Kim, Eungbin
通讯作者：
Kim, Eungbin
DOI：
10.1128/genomea.01038-17
发表时间：
2017-09-01
期刊：
GENOME ANNOUNCEMENTS
影响因子：
--
作者：
Furmanczyk, Ewa M.;Kaminski, Michal A.;Sobczak, Adam
通讯作者：
Sobczak, Adam
DOI：
10.1007/978-981-10-2555-6_2
发表时间：
2016-01-01
期刊：
MICROBIAL MODELS: FROM ENVIRONMENTAL TO INDUSTRIAL SUSTAINABILITY
影响因子：
--
作者：
Batista, Marcelo Bueno;Mueller-Santos, Marcelo;de Souza, Emanuel Maltempi
通讯作者：
de Souza, Emanuel Maltempi