Identification of JAK/STAT pathway regulators--insights from RNAi screens.
Identification of JAK/STAT pathway regulators--insights from RNAi screens.
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DOI：
10.1016/j.semcdb.2008.06.001
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发表时间：
2008-08
影响因子：
7.3
通讯作者：
Zeidler MP
中科院分区：
文献类型：
--
作者：
Müller P;Boutros M;Zeidler MP
虽然许多核心JAK/STAT途径的组成部分已被发现在果蝇通过经典的遗传学方法，途径调节剂的鉴定一直更具挑战性。最近，两个基于细胞的RNAi筛选JAK/STAT通路调节剂已经进行了使用双链RNA库靶向预测的果蝇转录组的大部分。虽然两个筛选鉴定了多个调节子，但两个数据集只有相对较少的基因座是共同的。在这里，我们比较两个屏幕，并讨论这些差异。尽管可能有许多因素起作用，但检测试剂盒设计的差异至关重要。低水平刺激有利于识别负性通路调节因子，高水平刺激有利于识别正性作用因子。最终，两种筛选的结果可能在很大程度上是互补的，并确定了一系列JAK/STAT通路活性的新候选调节剂，作为未来新研究方向的起点。
While many core JAK/STAT pathway components have been discovered in Drosophila via classical genetic approaches, the identification of pathway regulators has been more challenging. Recently two cell-based RNAi screens for JAK/STAT pathway regulators have been undertaken using libraries of double-stranded RNAs targeting a large proportion of the predicted Drosophila transcriptome. While both screens identified multiple regulators, only relatively few loci are common to both data sets. Here we compare the two screens and discuss these differences. Although many factors are likely to be contributory, differences in the assay design are of key importance. Low levels of stimulation favouring the identification of negative pathway regulators and high levels of stimulation favouring the identification of positively acting factors. Ultimately, the results from both screens are likely to be largely complementary and have identified a range of novel candidate regulators of JAK/STAT pathway activity as a starting point for new research directions in the future.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
12.3
作者：
Boutros M;Brás LP;Huber W
通讯作者：
Huber W
影响因子：
1.2
作者：
Bach, Erika A.;Ekas, Laura A.;Baeg, Gyeong-Hun
通讯作者：
Baeg, Gyeong-Hun
影响因子：
4.6
作者：
Brown, S;Hu, N;Hombría, JCG
通讯作者：
Hombría, JCG
DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
Clemens, JC;Worby, CA;Dixon, JE
通讯作者：
Dixon, JE