Strength and specificity of different gene promoters in oral cancer cells.
Strength and specificity of different gene promoters in oral cancer cells.
复制标题
口腔癌细胞中不同基因启动子的强度和特异性。
DOI：
10.1016/s1368-8375(99)00064-0
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Noonan,S
中科院分区：
文献类型：
--
作者：
Shillitoe,EJ;Noonan,S
Gene therapy of oral cancer will require expression of genes by promoters that are both powerful and relatively tumor specific. We compared the level of expression of a reporter gene from promoters of human cytomegalovirus (CMV), SV40 virus, mouse mammary tumor virus (MMTV), human papillomaviruses (HPV) types 16 and 18, and the human multi-drug-resistance gene (mdr1), in several lines of oral cancer cells. In the oral cancer cell line 686LN the rank order of expression levels was: CMV>SV40>HPV>mdr1>MMTV. Unlike in previous reports the mdr1 promoter was no more active in two cancer cell lines with mutations in the p53 gene than in two other lines with wild-type p53, and its expression level could not be increased by either doxorubicin or taxol. On the other hand, expression from the MMTV promoter was increased over 10-fold by the presence of 1 μM dexamethasone. Thus, by an appropriate choice of promoter and inducer a wide variety of expression levels, over a 3-log range, could be attained in 686LN cells. The oral cancer-specificity of each promoter was judged by comparing expression in the neuroblastoma line IMR32. The most specific promoters were those from papillomaviruses, which were up to 45 times more active in the oral cancer cells, and the least specific was the CMV promoter. In order to find if an HPV-derived promoter was sufficient to produce expression of a suicide phenotype the 686 promoter was cloned adjacent to the thymidine kinase gene of herpes simplex and the construct was expressed from an adenovirus vector. The vector reduced the growth of 686LN cells over a 5-day period by up to 32% when optimal concentrations of virus and ganciclovir were used. These data will be valuable in the design of new constructs for gene therapy of oral cancer.
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单纯疱疹病毒对人类口腔癌细胞的影响，以及突变病毒在口腔癌治疗中的潜在用途。
DOI：
10.1016/s1368-8375(98)00085-2
发表时间：
1999
期刊：
Oral oncology.
影响因子：
--
作者：
Shillitoe,EJ;Gilchrist,E;Pellenz,C;Murrah,V
通讯作者：
Murrah,V
影响因子：
10.5
作者：
LEASK, A;ROSENBERG, M;FUCHS, E
通讯作者：
FUCHS, E
影响因子：
3.4
作者：
Liu, JJ;Kao, WWY;Wilson, SE
通讯作者：
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影响因子：
11.2
作者：
R. Vile;I. Hart
通讯作者：
I. Hart
影响因子：
11.2
作者：
Myong Soo Kim;K. Shin;Jeong-Hwa Baek;H. Cherrick;No-Hee Park
通讯作者：
Myong Soo Kim;K. Shin;Jeong-Hwa Baek;H. Cherrick;No-Hee Park