Light- and electron-microscopic localization of basic fibroblast growth factor in adult rat retina.
Light- and electron-microscopic localization of basic fibroblast growth factor in adult rat retina.
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成年大鼠视网膜中碱性成纤维细胞生长因子的光和电子显微镜定位。
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Masahiro Sakanaka
中科院分区：
文献类型：
--
作者：
Akihiro Morimoto;S. Matsuda;Kunihiro Uryu;H. Fujita;N. Okumura;Masahiro Sakanaka
通过免疫印迹法对碱性成纤维细胞生长因子（bFGF）的抗血清进行了表征，并用于定位成年大鼠视网膜中的bFGF免疫反应性。用免疫印迹法检测视网膜粗匀浆时，显示出与bFGF相同分子量（18 kDa）的主带。光镜下免疫组化结果显示，bFGF主要分布于光感受器外节和外丛状层，外核层、内核层和节细胞层的部分细胞呈弱阳性。最强烈的bFGF-免疫反应，指出在神经胶质细胞（Müller）垂直取向薄的过程。脉络膜内也有免疫反应阳性的色素上皮细胞和间充质细胞。在电子显微镜下，外丛状层被发现含有免疫反应产物只在感光细胞的轴突终末形成突触与双极细胞的过程。bFGF-免疫反应性在成熟大鼠视网膜中的更广泛和不均匀的定位比以前证明的表明，内源性bFGF参与复杂的生理事件以外的视网膜再生和组织发生。
An antiserum against basic fibroblast growth factor (bFGF) was characterized by immunoblot and used to localize bFGF-immunoreactivity in the adult rat retina. Crude homogenate of the retina, when examined with immunoblotting, exhibited a main band with the same molecular weight (18kDa) as bFGF. Light microscopic immunohistochemistry demonstrated intense bFGF staining in the photoreceptor outer segments and the outer plexiform layer, and weak immunostaining in some cells of the outer nuclear layer, inner nuclear layer and ganglion cell layer. The most intense bFGF-immunoreactivity was noted in glial (Müller) cells with vertically oriented thin processes. There were also immunoreactive pigment epithelial cells and mesenchymal cells in the choroid. Under electron microscopy, the outer plexiform layer was found to contain immunoreactive products exclusively in the axon terminals of photoreceptor cells which formed synapses with bipolar cell processes. The more widespread and uneven localization of bFGF-immunoreactivity in the mature rat retina than previously demonstrated suggests that endogenous bFGF is involved in complex physiological events other than retinal regeneration and histogenesis.
影响因子：
56.9
作者：
DIMARIO, J;BUFFINGER, N;STROHMAN, RC
通讯作者：
STROHMAN, RC
成纤维细胞生长因子促进分离的海马神经元的存活并增强神经突的延伸。
DOI：
10.1073/pnas.83.9.3012
发表时间：
1986
影响因子：
11.1
作者：
Walicke,P;Cowan,WM;Ueno,N;Baird,A;Guillemin,R
通讯作者：
Guillemin,R