Glycan Imaging Mass Spectrometry: Progress in Developing Clinical Diagnostic Assays for Tissues, Biofluids, and Cells.
Glycan Imaging Mass Spectrometry: Progress in Developing Clinical Diagnostic Assays for Tissues, Biofluids, and Cells.
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DOI：
10.1016/j.cll.2021.03.005
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发表时间：
2021-06
影响因子：
1.7
通讯作者：
Drake RR
中科院分区：
文献类型：
--
作者：
Blaschke CRK;McDowell CT;Black AP;Mehta AS;Angel PM;Drake RR
蛋白质糖基化是一种非常普遍的翻译后修饰，涉及低聚糖的附着，最常见的是丝氨酸/苏氨酸残基（o -键糖基化）或天冬酰胺残基（n -键糖基化）。糖基化是一个高度调控的过程，超过300种酶以非模板驱动的方式参与其生物合成和加工。这是通过一系列连续的反应来完成的，这些反应涉及一系列广泛的糖转移酶，这些酶附着来自核苷酸糖供体的单糖，以及去除单个单糖的不同的糖苷酶。这些酶的表达、活性和定位对细胞的生理状态很敏感。据估计，2 n -链糖基化发生在超过一半的人类蛋白质上，在蛋白质折叠、分子运输、信号转导、细胞-细胞相互作用和许多其他过程中起着重要作用。3-6聚糖是细胞间相互作用的初始接触点之一，因此聚糖生物合成中的疾病变化可能比与基因突变和蛋白质相关的疾病变化更明显。在多种疾病中都发现了n -链糖基化的改变，包括但不限于癌症、炎症性关节炎、肝纤维化/肝硬化、精神分裂症、2型糖尿病、缺血性中风、帕金森病。特别是对于癌症，大多数fda批准的癌症生物标志物由血液中的循环糖蛋白或碳水化合物抗原组成。7,8,15 -18糖蛋白可以作为理想的生物标志物，因为它们通过活性途径从组织或血细胞进入循环系统
Protein glycosylation is a highly prevalent post-translational modification involving the attachment of oligosaccharides, most commonly to a serine/threonine residue (O-linked glycosylation) or an asparagine residue (N-linked glycosylation). Glycosylation is a highly regulated process with over 300 enzymes involved in their biosynthesis and processing in a non-template driven manner. This is done by a series of sequential reactions involving a wide array of glycotransferases that attach single monosaccharides from nucleotide sugar donors, as well as different glycosidases that remove individual monosaccharides. 1 The expression, activity, and localization of these enzymes are sensitive to the physiological state of the cell. Estimated to occur on over half of human proteins, 2 N-linked glycosylation has essential roles in protein folding, molecular trafficking, signal transduction, cell-cell interactions, and many other processes. 3-6 Glycans serve as one of the initial points of contact during cell to cell interactions, so therefore disease changes in glycan biosynthesis can be more apparent than disease related changes associated with gene mutations and proteins. 7 Alterations in N-linked glycosylation have been found in a variety of diseases, including but not limited to cancer, 8 inflammatory arthritis, 9 liver fibrosis/cirrhosis, 10 schizophrenia, 11 type 2 diabetes, 12 ischemic stroke, 13 and Parkinson’s disease. 14 Particularly for cancers, the majority of FDA-approved cancer biomarkers are comprised of circulating glycoproteins or carbohydrate antigens for measurement in blood. 7, 8, 15-18 Glycoproteins can be ideal biomarkers because they enter circulation from tissues or blood cells through active
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影响因子：
48
作者：
Chen, Songming;LaRoche, Tom;Haab, Brian B.
通讯作者：
Haab, Brian B.
影响因子：
3.1
作者：
Albrecht, Simone;Unwin, Louise;Rudd, Pauline M.
通讯作者：
Rudd, Pauline M.
影响因子：
29.4
作者：
Clerc, Florent;Novokmet, Mislav;Wuhrer, Manfred
通讯作者：
Wuhrer, Manfred
影响因子：
7.4
作者：
Angel, Peggi M.;Spraggins, Jeffrey M.;Baldwin, H. Scott;Caprioli, Richard
通讯作者：
Caprioli, Richard
影响因子：
5
作者：
Angel PM;Drake RR;Park Y;Clift CL;West C;Berkhiser S;Hardiman G;Mehta AS;Bichell DP;Su YR
通讯作者：
Su YR