The PRT6 N-degron pathway restricts VERNALIZATION 2 to endogenous hypoxic niches to modulate plant development.
The PRT6 N-degron pathway restricts VERNALIZATION 2 to endogenous hypoxic niches to modulate plant development.
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DOI：
10.1111/nph.16477
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发表时间：
2021-01
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影响因子：
--
通讯作者：
Gibbs DJ
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文献类型：
--
作者：
Labandera AM;Tedds HM;Bailey M;Sprigg C;Etherington RD;Akintewe O;Kalleechurn G;Holdsworth MJ;Gibbs DJ
VERNALIZATION2 （VRN2）是多梳抑制复合体2 （PRC2）的一个被子植物特异性亚基，是泛素介导的蛋白水解PRT6 N - degron途径中PCO分支的氧（O2）调控靶点。这种翻译后调控如何协调VRN2活性仍有待完全确定。本研究利用拟南芥生态型、突变体和转基因系来确定VRN2稳定性的控制如何影响其在植物发育过程中的功能。VRN2定位于地生组织和根组织的内源性缺氧区。在茎尖，VRN2根据光周期差异调节开花时间，而在侧根原基和根尖分生组织中，VRN2的存在负向调节根系结构。异位积累VRN2不会增强其对开花的影响，但会增强其对根生长的抑制作用。在开花晚春化依赖的生态型中，VRN2只有在低温下蛋白水解受到抑制时才在分生组织外活跃，因为它的功能需要低温触发的其他PRC2亚基和辅助因子的增加。我们得出结论，VRN2对O2敏感的N - degron具有双重功能，将VRN2限制在分生组织和原基中，在那里它具有特定的发育作用，同时也允许在分生组织外根据环境线索广泛积累，从而导致其他功能。另见Sasidharan等人对这篇文章的评论，229:5-7。
VERNALIZATION2 (VRN2), an angiosperm‐specific subunit of the polycomb repressive complex 2 (PRC2), is an oxygen (O2)‐regulated target of the PCO branch of the PRT6 N‐degron pathway of ubiquitin‐mediated proteolysis. How this post‐translational regulation coordinates VRN2 activity remains to be fully established. Here we use Arabidopsis thaliana ecotypes, mutants and transgenic lines to determine how control of VRN2 stability contributes to its functions during plant development. VRN2 localizes to endogenous hypoxic regions in aerial and root tissues. In the shoot apex, VRN2 differentially modulates flowering time dependent on photoperiod, whilst its presence in lateral root primordia and the root apical meristem negatively regulates root system architecture. Ectopic accumulation of VRN2 does not enhance its effects on flowering, but does potentiate its repressive effects on root growth. In late‐flowering vernalization‐dependent ecotypes, VRN2 is only active outside meristems when its proteolysis is inhibited in response to cold exposure, as its function requires concomitant cold‐triggered increases in other PRC2 subunits and cofactors. We conclude that the O2‐sensitive N‐degron of VRN2 has a dual function, confining VRN2 to meristems and primordia, where it has specific developmental roles, whilst also permitting broad accumulation outside of meristems in response to environmental cues, leading to other functions. See also the Editorial on this article by Sasidharan et al., 229: 5–7.
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