Insight into the potential antineoplastic mechanism of phycocyanin in non-small cell lung carcinoma A549 cells based on micro-RNA sequencing
Insight into the potential antineoplastic mechanism of phycocyanin in non-small cell lung carcinoma A549 cells based on micro-RNA sequencing
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基于micro-RNA测序揭示藻蓝蛋白在非小细胞肺癌A549细胞中的潜在抗肿瘤机制
DOI：
10.1016/j.jff.2020.104175
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发表时间：
2020-11
影响因子：
5.6
通讯作者：
Baoguo Sun
中科院分区：
文献类型：
--
作者：
Shuai Hao;Yuanpu Liu;Shuang Li;Jing Wang;Lei Zhao;Chengtao Wang;Baoguo Sun
藻蓝蛋白是一种海洋功能性食品添加剂，具有良好的抗肿瘤作用。然而，其在非小细胞肺癌（NSCLC）中的作用机制尚不清楚。本研究首次采用高通量micro-RNA测序技术分析了A549细胞中受藻蓝蛋白调控的关键miRNAs。结果显示，对照组和藻蓝蛋白处理组细胞中共有61个miRNAs差异表达，其中18个上调，43个下调。随后，预测了483个验证的靶基因。蛋白-蛋白相互作用分析显示，EGFR、CDK 2、POLR 2 E、TP 53和MED 28等靶基因在网络中占有很高的位置，预测这些基因受miR-1249- 5 p、miR-1231、miR-547- 5 p和miR-93- 5 p的调控。此外，qRT-PCR显示差异miRNA的表达趋势与miRNA-seq结果高度一致。因此，本研究为进一步了解藻蓝蛋白的调控机制，尤其是miRNA的调控机制提供了新的思路，也为NSCLC的治疗奠定了理论基础。
Phycocyanin is a type of marine functional food additive, exerting good antitumor property. However, its antineoplastic mechanism in non-small cell lung cancer (NSCLC) remains unclear. In this work, a high-throughput micro-RNA sequencing was employed to analyze key miRNAs regulated by phycocyanin in A549 cells for the first time. Results showed that a total of 61 miRNAs were differentially expressed between control and phycocyanin-treated cells, including 18 up-regulated and 43 down-regulated miRNAs. Subsequently, 483 validated target genes were predicted. Strikingly, protein–protein interaction analysis showed that target genes like EGFR, CDK2, POLR2E, TP53, and MED28 occupied high degrees in networks, which were predicted regulated by miR-1249-5p, miR-1231, miR-547-5p, and miR-93-5p. Furthermore, qRT-PCR showed strong consistent expression trends of differential miRNAs with miRNA-seq results. Consequently, this study significantly advances our understanding of the regulatory mechanisms of phycocyanin, especially with regard to miRNAs, and also lays a theoretical foundation for the treatment of NSCLC.
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