Analysis of the CRISPR-Cas system in bacteriophages active on epidemic strains of Vibrio cholerae in Bangladesh.
Analysis of the CRISPR-Cas system in bacteriophages active on epidemic strains of Vibrio cholerae in Bangladesh.
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DOI：
10.1038/s41598-017-14839-2
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发表时间：
2017-11-01
影响因子：
4.6
通讯作者：
Faruque SM
中科院分区：
文献类型：
--
作者：
Naser IB;Hoque MM;Nahid MA;Tareq TM;Rocky MK;Faruque SM
CRISPR-Cas（成簇规则散布的短回文重复序列 - CRISPR 相关蛋白）是参与防御噬菌体的微生物核酸酶系统。细菌还通过宿主噬菌体诱导染色体岛（PICI）来抵抗噬菌体，从而阻止噬菌体繁殖。引起霍乱流行的霍乱弧菌与环境中和霍乱患者体内的众多噬菌体相互作用。尽管 CRISPR-Cas 系统通常由细菌和古细菌携带，但最近发现霍乱弧菌特异性 ICP1 噬菌体具有 CRISPR-Cas 系统，该系统可灭活霍乱弧菌中的 PICI 样元件 (PLE)。我们分析了孟加拉国季节性霍乱流行期间分离的一系列噬菌体和霍乱弧菌，以研究噬菌体编码的 CRISPR-Cas 系统的分布和最新进化。鉴定出五种不同但相关的携带 CRISPR-Cas 系统的噬菌体，以及可能的 CRISPR-Cas 阴性祖噬菌体。此外，发现噬菌体中的 CRISPR 阵列是通过获取针对霍乱弧菌菌株携带的 PLE 不同区域的新间隔区而进化的，从而使噬菌体能够在 PLE 阳性菌株上有效生长。我们的结果表明，一场持续的军备竞赛涉及霍乱弧菌噬菌体抗性的遗传决定因素，以及霍乱弧菌及其噬菌体共同进化中噬菌体编码的 CRISPR-Cas 系统，这可能是由于它们在霍乱季节性流行期间增强的相互作用而促进的。
CRISPR-Cas (clustered regularly interspersed short palindromic repeats-CRISPR-associated proteins) are microbial nuclease systems involved in defense against phages. Bacteria also resist phages by hosting phage-inducible chromosomal islands (PICI) which prevent phage reproduction. Vibrio cholerae which causes cholera epidemics, interacts with numerous phages in the environment and in cholera patients. Although CRISPR-Cas systems are usually carried by bacteria and archea, recently V. cholerae specific ICP1 phages were found to host a CRISPR-Cas system that inactivates PICI-like elements (PLE) in V. cholerae. We analyzed a collection of phages and V. cholerae isolated during seasonal cholera epidemics in Bangladesh, to study the distribution, and recent evolution of the phage-encoded CRISPR-Cas system. Five distinct but related phages carrying the CRISPR-Cas system, and possible CRISPR-Cas negative progenitor phages were identified. Furthermore, CRISPR arrays in the phages were found to have evolved by acquisition of new spacers targeting diverse regions of PLEs carried by the V. cholerae strains, enabling the phages to efficiently grow on PLE positive strains. Our results demonstrate a continuing arms-race involving genetic determinants of phage-resistance in V. cholerae, and the phage-encoded CRISPR-Cas system in the co-evolution of V. cholerae and its phages, presumably fostered by their enhanced interactions during seasonal epidemics of cholera.
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