A light-sensing system in the common ancestor of the fungi.
A light-sensing system in the common ancestor of the fungi.
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DOI：
10.1016/j.cub.2022.05.034
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发表时间：
2022-07-25
期刊：
影响因子：
9.2
通讯作者：
Richards, Thomas A.
中科院分区：
文献类型：
--
作者：
Galindo, Luis Javier;Milner, David S.;Gomes, Suely Lopes;Richards, Thomas A.
不同的感光器官（即，眼睛）在动物中进化。有趣的是，在真核微生物中发现了几种亚细胞类似物。所有这些系统都有一个共同的“配方”：一个遮光或耐火表面与富含I型视紫红质的膜层并列。在真菌中，几个谱系已被证明使用多种非同源光响应蛋白来检测光。然而，这些系统并不与一个眼点样的细胞器相关联，只有一个例外是在动孢子菌Blastocladiella emersonii（Be）中发现的。Be拥有以下配方的两个元素：由脂质填充结构组成的眼点（通常称为侧体复合体[SBC]），与富含由I型多核苷酸组成的基因融合蛋白的膜共定位。（微生物）视紫红质和鸟苷酸环化酶酶结构域（CyclOp-fusion protein）。在这里，我们确定同源途径组件在四个壶菌目（壶菌目，Synchytriales，Rhizophydiales和Monoblepharidiales）。为了进一步探索真菌游动孢子及其脂质细胞器的结构，我们回顾了电子显微镜数据（例如，巴尔和哈特曼和Reichle和富勒的工作），并进行四种携带CyclOps的游动孢子真菌物种的荧光显微镜成像，显示存在多种候选的眼点-细胞骨架超微结构系统。然后，我们评估了真菌中典型光感受器的存在，并推断最后一个共同的真菌祖先能够使用各种系统（包括蓝绿光检测）感知一系列波长的光。我们的数据意味着，独立于真菌的生命树是如何扎根的，一个CyclOp-organelle光感知系统的装置是真菌的祖先特征。多种多样的有鞭毛的真菌拥有CyclOp光响应电路相同的真菌拥有亚细胞设备来建立基于脂质的眼点真菌的最后共同祖先拥有CyclOp眼点系统祖先真菌可以看到彩虹的光波长Galindo等人。表明控制游泳行为的光响应电路存在于各种真菌中。该回路与充满脂质的亚细胞体协调运作。分歧真菌具有相似的细胞体。系统发育表明，这种真菌祖先拥有这种光感应系统，沿着的还有其他光感受器。
Diverse light-sensing organs (i.e., eyes) have evolved across animals. Interestingly, several subcellular analogs have been found in eukaryotic microbes. All of these systems have a common “recipe”: a light occluding or refractory surface juxtaposed to a membrane-layer enriched in type I rhodopsins. In the fungi, several lineages have been shown to detect light using a diversity of non-homologous photo-responsive proteins. However, these systems are not associated with an eyespot-like organelle with one exception found in the zoosporic fungus Blastocladiella emersonii (Be).Be possesses both elements of this recipe: an eyespot composed of lipid-filled structures (often called the side-body complex [SBC]), co-localized with a membrane enriched with a gene-fusion protein composed of a type I (microbial) rhodopsin and guanylyl cyclase enzyme domain (CyclOp-fusion protein). Here, we identify homologous pathway components in four Chytridiomycota orders (Chytridiales, Synchytriales, Rhizophydiales, and Monoblepharidiales). To further explore the architecture of the fungal zoospore and its lipid organelles, we reviewed electron microscopy data (e.g., the works of Barr and Hartmann and Reichle and Fuller) and performed fluorescence-microscopy imaging of four CyclOp-carrying zoosporic fungal species, showing the presence of a variety of candidate eyespot-cytoskeletal ultrastructure systems. We then assessed the presence of canonical photoreceptors across the fungi and inferred that the last common fungal ancestor was able to sense light across a range of wavelengths using a variety of systems, including blue-green-light detection. Our data imply, independently of how the fungal tree of life is rooted, that the apparatus for a CyclOp-organelle light perception system was an ancestral feature of the fungi. A wide diversity of flagellated fungi possess the CyclOp light response circuit The same fungi possess the subcellular equipment to build lipid-based eyespots The last common ancestor of fungi possessed the CyclOp eyespot system The ancestral fungus could see a rainbow of light wavelengths Galindo et al. demonstrate that a light response circuit, which controls swimming behavior, is present across diverse fungi. The circuit functions in coordination with a lipid-filled subcellular body. Divergent fungi possess similar cellular bodies. Phylogenies show that the ancestral fungus possessed this light-sensing system, along with other photoreceptors.
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影响因子：
14.9
作者：
Clark K;Karsch-Mizrachi I;Lipman DJ;Ostell J;Sayers EW
通讯作者：
Sayers EW
DOI：
10.1099/00221287-66-2-171
发表时间：
1971-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
FULLER, MS;OLSON, LW
通讯作者：
OLSON, LW
影响因子：
64.8
作者：
Gavelis, Gregory S.;Hayakawa, Shiho;Leander, Brian S.
通讯作者：
Leander, Brian S.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ