A multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in Saccharomyces cerevisiae.

A multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in Saccharomyces cerevisiae.
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基于多物种的分类微阵列揭示了酿酒酵母的种间杂交和基因渗入。

DOI:
10.1111/j.1567-1364.2008.00464.x
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发表时间:
2009-02
影响因子:
3.2
通讯作者:
McCusker, John H.
McCusker, John H.
中科院分区:
生物学4区
文献类型:
--
作者:
Muller, Ludo A. H.;McCusker, John H.

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设计了一种基于多物种的分类微阵列,其靶向酿酒酵母、奇异酵母、S. mikatae、S. bayanus、S. kudriavzevii、Naumoviacastellii、Lachancea kluyveri和光滑念珠菌中不同直系同源基因的编码序列,以允许鉴定这些物种的分离株及其种间杂种。对几种酵母菌属物种和种间杂交体的分离物的分析证明了微阵列能够根据这些酵母菌的特定杂交模式来区分这些酵母菌。随后对不同生态和地理背景的 183 个假设的酿酒酵母分离株进行分析,发现一种错误分类的 S. bayanus 或 S. uvarum 分离株以及四种非整倍体种间杂种,其中一种在酿酒酵母和 S. bayanus 之间,另一种在 S. cerevisiae 和 S. kudriavzevii 之间。此外,这种微阵列设计允许在三个不同的酿酒酵母分离株的基因组中检测多个渐渗的奇异酵母 DNA 片段。这些结果显示了基于多物种的微阵列作为鉴定物种和种间杂种的分类学工具的能力,以及它们提供种间杂种和重组体的更详细表征的能力。
A multi-species based taxonomic microarray targeting coding sequences of diverged orthologous genes in Saccharomyces cerevisiae, S. paradoxus, S. mikatae, S. bayanus, S. kudriavzevii, Naumovia castellii, Lachancea kluyveri and Candida glabrata was designed to allow identification of isolates of these species and their interspecies hybrids. Analysis of isolates of several Saccharomyces species and interspecies hybrids demonstrated the ability of the microarray to differentiate these yeasts on the basis of their specific hybridization patterns. Subsequent analysis of 183 supposed S. cerevisiae isolates of various ecological and geographical backgrounds revealed one misclassified S. bayanus or S. uvarum isolate and four aneuploid interspecies hybrids, one between S. cerevisiae and S. bayanus and three between S. cerevisiae and S. kudriavzevii. Furthermore, this microarray design allowed the detection of multiple introgressed S. paradoxus DNA fragments in the genomes of three different S. cerevisiae isolates. These results show the power of multi-species based microarrays as taxonomic tools for the identification of species and interspecies hybrids, and their ability to provide a more detailed characterization of interspecies hybrids and recombinants.
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