Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L.
Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L.
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DOI：
10.1093/dnares/dsq030
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
Fukui K
中科院分区：
文献类型：
--
作者：
Sato S;Hirakawa H;Isobe S;Fukai E;Watanabe A;Kato M;Kawashima K;Minami C;Muraki A;Nakazaki N;Takahashi C;Nakayama S;Kishida Y;Kohara M;Yamada M;Tsuruoka H;Sasamoto S;Tabata S;Aizu T;Toyoda A;Shin-i T;Minakuchi Y;Kohara Y;Fujiyama A;Tsuchimoto S;Kajiyama S;Makigano E;Ohmido N;Shibagaki N;Cartagena JA;Wada N;Kohinata T;Atefeh A;Yuasa S;Matsunaga S;Fukui K
采用传统的Sanger法和新一代多重测序法对麻疯树（Jatropha curcas）进行全基因组测序。由此获得的非冗余序列总长度为285 858 490 bp，由120 586个contigs和29 831个singlets组成。它们占基因含区约95%，平均G + C含量为34.3%。共推导出40929个蛋白质编码基因的完整和部分结构。与其他植物基因的比较表明，1529个（4%）的蛋白质编码基因是大戟科特有的。蓖麻基因组具有高度的微共合性，与大豆和拟南芥基因组的微共合性较低。在基因组测序的同时，对来自叶片和愈伤组织的cdna进行焦磷酸测序，共获得21 225个单基因数据。利用基因组序列数据开发的微卫星标记对来自世界各地的12个麻树品系进行多态性分析，以估计其遗传多样性。本文所提供的基因组序列和相关信息有望成为加速麻瓜基础研究和应用研究的宝贵资源，特别是在生物燃料生产等与环境有关的研究领域。更多关于基因组序列和DNA标记的信息可在。
The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ∼95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at .
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