Early Changes of Articular Cartilage and Subchondral Bone in The DMM Mouse Model of Osteoarthritis.
Early Changes of Articular Cartilage and Subchondral Bone in The DMM Mouse Model of Osteoarthritis.
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DMM小鼠骨关节炎模型中关节软骨和软骨下骨的早期变化
DOI：
10.1038/s41598-018-21184-5
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发表时间：
2018-02-12
影响因子：
4.6
通讯作者：
Cai D
中科院分区：
文献类型：
--
作者：
Fang H;Huang L;Welch I;Norley C;Holdsworth DW;Beier F;Cai D
为观察DMM小鼠骨性关节炎模型早期关节软骨和软骨下骨的变化，分别于术后2、5、10周处死小鼠，进行DMM或假手术。分别采用T步态分析、计算机断层扫描、甲苯胺蓝、天狼星红和抗酒石酸酸性磷酸酶(TRAP)染色观察步态、关节形态、软骨、软骨、胶原组织和破骨细胞活性。结果显示，骨性关节炎的进展超过10周的时间。仅在术后10周出现步态不一致。术后2周形成骨赘。DMM组2周时BMD与假手术组比较无统计学差异，但BV/TV值明显高于假手术组。在DMM小鼠手术后5周和10周，骨密度明显增加。TRAP染色显示5周和10周时DMM关节骨赘形成部位破骨细胞活性增强。这些结果表明，在该小鼠DMM模型中，软骨下骨转换发生的时间可能早于2周。在DMM小鼠中，步态差异仅出现在骨性关节炎的晚期。值得注意的是，一些DMM小鼠可能发生膝盖骨脱位，并在受累的膝关节中引起不同类型的骨性关节炎。
To examine the early changes of articular cartilage and subchondral bone in the DMM mouse model of osteoarthritis, mice were subjected to DMM or SHAM surgery and sacrificed at 2-, 5- and 10-week post-surgery. Catwalk gait analyses, Micro-Computed Tomography, Toluidine Blue, Picrosirius Red and Tartrate-Resistant Acid Phosphatase (TRAP) staining were used to investigate gait patterns, joint morphology, subchondral bone, cartilage, collagen organization and osteoclasts activity, respectively. Results showed OA progressed over 10-week time-course. Gait disparity occurred only at 10-week post-surgery. Osteophyte formed at 2-week post-surgery. BMDs of DMM showed no statistical differences comparing to SHAM at 2 weeks, but BV/TV is much higher in DMM mice. Increased BMD was clearly found at 5- and 10-week post-surgery in DMM mice. TRAP staining showed increased osteoclast activity at the site of osteophyte formation of DMM joints at 5- and 10-week time points. These results showed that subchondral bone turnover might occurred earlier than 2 weeks in this mouse DMM model. Gait disparity only occurred at later stage of OA in DMM mice. Notably, patella dislocation could occur in some of the DMM mice and cause a different pattern of OA in affected knee.
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影响因子：
3.7
作者：
Zhang RC;Lin Y;Yue M;Li Q;Zhang XF;Liu X;Chi H;Chai YF;Wang M
通讯作者：
Wang M
影响因子：
13.3
作者：
Ratneswaran, A.;LeBlanc, E. A.;Beier, F.
通讯作者：
Beier, F.
影响因子：
4.1
作者：
Usmani, Shirine E.;Pest, Michael A.;Beier, Frank
通讯作者：
Beier, Frank
影响因子：
7
作者：
Botter, S. M.;Glasson, S. S.;van Osch, G. J. V. M.
通讯作者：
van Osch, G. J. V. M.
影响因子：
2.8
作者：
Newberry, WN;Zukosky, DK;Haut, RC
通讯作者：
Haut, RC