Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets.
Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets.
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DOI：
10.1016/j.virol.2015.03.024
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发表时间：
2015-08
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Chen Q;Gauger P;Stafne M;Thomas J;Arruda P;Burrough E;Madson D;Brodie J;Magstadt D;Derscheid R;Welch M;Zhang J
猪DeltaCoronaVirus(PDCoV)于2009-2010年在香港首次发现，并于2014年2月首次在美国猪群中报告。然而，目前尚缺乏除聚合酶链式反应以外的诊断工具，且尚不能满足科赫׳S的假设来确认冠状病毒的致病潜力。在本研究中，我们制备了PDCoV多肽特异性兔抗血清，并将其用于免疫荧光和免疫组织化学检测以辅助PDCoV的诊断。用空斑纯化的PDCoV细胞分离株(每头猪3×10~4个TCID50)经口接种5日龄仔猪，研究了PDCoV的致病性和致病性。接种PDCoV的仔猪出现轻至中度腹泻，接种后2~7d，直肠拭子中的病毒量增加，免疫组织化学染色证实病毒抗原出现小肠大体和微观病变。本研究从实验上证实了PDCoV对新生仔猪的致病性，并对其致病机理进行了研究。
Porcine deltacoronavirus (PDCoV) was first identified in Hong Kong in 2009–2010 and reported in United States swine for the first time in February 2014. However, diagnostic tools other than polymerase chain reaction for PDCoV detection were lacking and Koch׳s postulates had not been fulfilled to confirm the pathogenic potential of PDCoV. In the present study, PDCoV peptide-specific rabbit antisera were developed and used in immunofluorescence and immunohistochemistry assays to assist PDCoV diagnostics. The pathogenicity and pathogenesis of PDCoV was investigated following orogastric inoculation of 5-day-old piglets with a plaque-purified PDCoV cell culture isolate (3×104 TCID50 per pig). The PDCoV-inoculated piglets developed mild to moderate diarrhea, shed increasing amount of virus in rectal swabs from 2 to 7 days post inoculation, and developed macroscopic and microscopic lesions in small intestines with viral antigen confirmed by immunohistochemistry staining. This study experimentally confirmed PDCoV pathogenicity and characterized PDCoV pathogenesis in neonatal piglets.
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影响因子：
11.8
作者：
Wang L;Byrum B;Zhang Y
通讯作者：
Zhang Y
DOI：
10.1128/genomea.00218-14
发表时间：
2014-04-10
期刊：
Genome announcements
影响因子：
--
作者：
Marthaler D;Jiang Y;Collins J;Rossow K
通讯作者：
Rossow K
影响因子：
3.3
作者：
Madson, D. M.;Magstadt, D. R.;Yoon, K. J.
通讯作者：
Yoon, K. J.
影响因子：
5.4
作者：
Woo, Patrick C. Y.;Lau, Susanna K. P.;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
11.8
作者：
Marthaler, Douglas;Raymond, Lindsey;Rovira, Albert
通讯作者：
Rovira, Albert