Spx, the central regulator of the heat and oxidative stress response in B. subtilis, can repress transcription of translation‐related genes
Spx, the central regulator of the heat and oxidative stress response in B. subtilis, can repress transcription of translation‐related genes
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Spx 是枯草芽孢杆菌热和氧化应激反应的中心调节因子，可以抑制翻译相关基因的转录
DOI：
10.1111/mmi.14171
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发表时间：
2019
影响因子：
3.6
通讯作者：
Turgay
中科院分区：
文献类型：
--
作者：
Schäfer;Sudzinová;Hantke;Krásný;Turgay
Spx是一种枯草杆菌转录因子，与RNA聚合酶的α亚基相互作用。它可以激活巯基应激反应调节子并干扰许多发育过程的激活。在这里，我们表明Spx是通过上调相关应激反应基因来协调热休克反应的核心参与者，正如比较转录组学实验所揭示的那样。此外，这些实验揭示了Spx抑制翻译相关基因转录的潜力。通过体内和体外实验，证实了Spx对rRNA的转录有抑制作用。这种抑制主要依赖于UP元件和RNA聚合酶的α亚基。然而，Spx同时上调胁迫基因的活性，而不是抑制翻译相关基因，对于在施加的胁迫条件下介导胁迫反应和抗生素耐受性至关重要。观察到的抑制活性可能通过干扰翻译的额外应激反应过程在体内得到补偿。然而，在高细胞Spx水平的条件下，Spx对限制翻译的影响变得明显。有趣的是，我们观察到一个稳定期细胞亚群含有升高的Spx水平，这可能有助于生长抑制和该亚群在生长过程中的持续性行为。
Spx is aBacillus subtilistranscription factor that interacts with the alpha subunits of RNA polymerase. It can activate the thiol stress response regulon and interfere with the activation of many developmental processes. Here, we show that Spx is a central player orchestrating the heat shock response by up‐regulating relevant stress response genes as revealed by comparative transcriptomic experiments. Moreover, these experiments revealed the potential of Spx to inhibit transcription of translation‐related genes. Byin vivoandin vitroexperiments, we confirmed that Spx can inhibit transcription from rRNA. This inhibition depended mostly on UP elements and the alpha subunits of RNA polymerase. However, the concurrent up‐regulation activity of stress genes by Spx, but not the inhibition of translation related genes, was essential for mediating stress response and antibiotic tolerance under the applied stress conditions. The observed inhibitory activity might be compensatedin vivoby additional stress response processes interfering with translation. Nevertheless, the impact of Spx on limiting translation becomes apparent under conditions with high cellular Spx levels. Interestingly, we observed a subpopulation of stationary phase cells that contains raised Spx levels, which may contribute to growth inhibition and a persister‐like behaviour of this subpopulation during outgrowth.
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影响因子：
14.9
作者：
Michna RH;Zhu B;Mäder U;Stülke J
通讯作者：
Stülke J
影响因子：
3.7
作者：
Murayama S;Ishikawa S;Chumsakul O;Ogasawara N;Oshima T
通讯作者：
Oshima T
大肠杆菌的温度敏感性生长和耐热性降低与 relA 突变相关
影响因子：
3.2
作者：
Xiaoming Yang;E. Ishiguro
通讯作者：
E. Ishiguro
DOI：
10.1006/bbrc.2002.6559
发表时间：
2002-03-08
影响因子：
3.1
作者：
Griffith, KL;Shah, IM;Wolf, RE
通讯作者：
Wolf, RE
影响因子：
5.6
作者：
RAO, L;ROSS, W;GOURSE, RL
通讯作者：
GOURSE, RL